{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:02:56Z","timestamp":1790611376077,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The PCR for Antigen Receptor Rearrangement (PARR) assay assesses clonality of a lymphoid population. Studies suggest PARR has decreased sensitivity for detecting clonality in canine plasma cell tumors (PCT) compared to lymphoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Assess sensitivity of PARR assays targeting the immunoglobulin heavy chain (IGH), immunoglobulin lambda light chain (IGL), and kappa deleting element (Kde) loci in canine PCT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Canine cases included 35 PCTs (multiple myeloma and extramedullary, cutaneous or oral PCTs), 5 non\u2010PCT foil cases, and 40 B\u2010cell lymphoma cases. PCT diagnoses were confirmed via CD3, PAX5, and MUM1 immunolabeling, and some additionally had histopathology, serum or urine protein electrophoresis, or immunofixation. Nodal B\u2010cell lymphomas were diagnosed by flow cytometry. Routine PARR (targeting IGH V\u2010D\u2010J and D\u2010J rearrangements) and extended PARR (targeting additional IGH genes and IGL and Kde rearrangements) were performed on cytologic specimens (PCT and foil cases) and flow cytometry aspirates (lymphoma cases). Diagnostic sensitivity was calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Across both PARR assays, every PCT case with sufficient sample was interpreted as clonal and no foil cases were clonal. The routine PARR assay detected a clonal immunoglobulin rearrangement in 26\/35 PCT cases (74.3%; 95% CI 56.7%\u201387.5%). The extended assay detected immunoglobulin gene clonality in 33\/35 PCT cases (94.3%; 95% CI 80.8%\u201399.3%). 40\/40 lymphoma cases were clonal in both the routine and extended PARR assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The combined PARR assays used in this study, evaluating multiple IG loci, detected clonality in all PCTs tested, but routine PARR (targeting IGH) was less sensitive for detecting PCT clonality compared to B\u2010cell lymphomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.70036","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T08:44:10Z","timestamp":1752569050000},"page":"281-291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Diagnostic Sensitivity of the <scp>PCR<\/scp> for Antigen Receptor Rearrangement (<scp>PARR<\/scp>) Assay for Canine Plasma Cell Tumors"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1435-8532","authenticated-orcid":false,"given":"Emily D.","family":"Rout","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kacie","family":"Seymour","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Burnett","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cora","family":"Contreras","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne C.","family":"Avery","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4904-8788","authenticated-orcid":false,"given":"A Russell","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Pathology Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.40-1-32"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(99)00051-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2005.10.009"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.09.014"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.10.008"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2019.109903"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15913"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2022.110498"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15485"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2015.03.005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2022.07.009"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-02-069625"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.09.010"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/10406387221139799"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12940"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12993"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15624"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400904"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/fampra\/cmh103"},{"key":"e_1_2_7_22_1","first-page":"4841","article-title":"Statistical Notes for Clinical Researchers: Chi\u2010Squared Test and Fisher's Exact Test","volume":"74","author":"Kim H. 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