{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:59:47Z","timestamp":1781107187943,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A pre\u2010transfusion blood crossmatch is recommended to ensure RBC compatibility for previously transfused and transfusion\u2010na\u00efve cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To compare 3 crossmatch methods and anti\u2010feline globulin (AFG) enhancement for determining RBC compatibility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Prospective study. Major crossmatches were performed using serum from 68 \u201crecipient\u201d cats and RBCs from 44 \u201cdonor\u201d cats. Crossmatch methods evaluated include laboratory tube and gel column without and with AFG enhancement and an in\u2010clinic AFG\u2010enhanced immunochromatographic strip.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Tube and gel crossmatches were performed for 211 and strip crossmatches for 133 recipient\u2010donor pairs. RBC incompatibilities were noted in at least 1 crossmatch method for 123 recipient\u2010donor pairs. For determination of the degree of incompatibility, there was a correlation between standard and AFG\u2010enhanced crossmatches for both tube and gel (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), standard tube and gel (<jats:italic>p<\/jats:italic>\u2009=\u20090.002), and AFG\u2010enhanced tube and gel (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Twelve of 46 and 22 of 113 recipient\u2010donor pairs deemed incompatible with tube and gel, respectively, had RBC agglutination noted only with the addition of AFG. RBC incompatibilities were noted on 15 strips, 4 of which were type A\u2010B mismatched. Odds of detecting RBC incompatibility using strip were 2.9 (<jats:italic>p<\/jats:italic>\u2009=\u20090.001) and 6.1 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) times greater with each unit increase in the degree of incompatibility detected by standard and AFG\u2010enhanced tube, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The clinical relevance of any observed <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> incompatibilities, other than known A\u2010B mismatches, is unknown. While the strip crossmatch is simple to perform and interpret, it might not be sufficiently sensitive to detect weaker <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> incompatibilities.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.70039","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T08:23:28Z","timestamp":1755851008000},"page":"230-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of Three Feline Crossmatch Methods\u2014Tube, Gel Column, and Immunochromatographic Strip"],"prefix":"10.1111","volume":"54","author":[{"given":"Victoria K.","family":"DiCiccio","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecka S.","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Weinstein","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Fromm","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Marryott","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4508-9980","authenticated-orcid":false,"given":"Mary Beth","family":"Callan","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine University of Pennsylvania  Philadelphia Pennsylvania 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