{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T18:38:26Z","timestamp":1758220706557,"version":"3.44.0"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In small animals, serum total thyroxine concentration (TT<jats:sub>4<\/jats:sub>) is an important parameter used to assess thyroid function in patients. In rabbits, no information is available on the prevalence of thyroid disease and its diagnosis by laboratory methods. The aim of this study was to establish a device\u2010 and method\u2010specific reference interval for TT<jats:sub>4<\/jats:sub> in clinically healthy rabbits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives and Methods<\/jats:title><jats:p>TT<jats:sub>4<\/jats:sub> concentrations were determined by chemiluminescence assay (CLIA) in 120 clinically healthy pet rabbits (median age 36\u2009months); the study population was divided into age groups (Group 1: 7\u201324\u2009months, Group 2: 25\u201359\u2009months, Group 3: &gt;\u200960\u2009months). The pet rabbits were kept indoors, outdoors, and indoor\/outdoor. The reference interval (95 percentile) was calculated using SPSS (IBM, version 28.0) according to the standard of the ASVCP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A median TT<jats:sub>4<\/jats:sub> concentration of 0.8\u2009\u03bcg\/dL (10.28\u2009nmol\/L) and a reference interval of 0.6\u20131.98\u2009\u03bcg\/dL (7.71\u201325.44\u2009nmol\/L) were determined. No statistically significant differences were found for sex (<jats:italic>p<\/jats:italic>\u2009=\u20090.889), age (<jats:italic>p<\/jats:italic>\u2009=\u20090.176), and between animals in indoor and outdoor housing (<jats:italic>p<\/jats:italic>\u2009=\u20090.967). Highly significant differences were observed between indoor and indoor\/outdoor housing (<jats:italic>p<\/jats:italic>\u2009=\u20090.002) and significant differences between outdoor and indoor\/outdoor housing (<jats:italic>p<\/jats:italic>\u2009=\u20090.006).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Concentrations of TT<jats:sub>4<\/jats:sub> in pet rabbits measured by chemiluminescence appear to be independent of sex and age. Laboratory and device\u2010specific reference intervals for pet rabbits should be used to avoid the influence of different methods, devices, calculation methods, and sample size.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.70041","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T12:29:16Z","timestamp":1756729756000},"page":"251-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serum Total Thyroxine Concentrations in Clinically Healthy Pet Rabbits"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9908-1048","authenticated-orcid":false,"given":"Jana","family":"Liebscher","sequence":"first","affiliation":[{"name":"Laboklin GmbH Und Co. KG  Bad Kissingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Laboklin GmbH Und Co. KG  Bad Kissingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.22233\/9781910443255.17"},{"key":"e_1_2_6_3_1","first-page":"497","volume-title":"Veterinary Hematology and Clinical Chemistry","author":"Meuten D.","year":"2012"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.15654\/TPK\u2010161137"},{"key":"e_1_2_6_5_1","first-page":"571","volume-title":"Veterinary Hematology and Clinical Chemistry","author":"Campbell T. W.","year":"2012"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879700900307"},{"key":"e_1_2_6_7_1","unstructured":"K.Friedrichs K.Barnhart J.Blanco et\u00a0al. \u201cASVCP Quality Assurance and Laboratory Standards Committee (QALS) Guidelines for the Determination of Reference Intervals in Veterinary Species and Other Related Topics: SCOPE \u201d accessed April 24 2023 https:\/\/cdn.ymaws.com\/www.asvcp.org\/resource\/resmgr\/QALS\/Other_Publications\/RI_Guidelines_For_ASVCP_webs.pdf."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo\u2010103\u20104\u20101434"},{"key":"e_1_2_6_9_1","unstructured":"J.Brandao M.Rick andT.Tully \u201cMeasurement of Serum Free and Total Thyroxine and Triiodothyronine Concentrations in Rabbits \u201dProceedings ExoticsCon Portland USA August 27\u2013Spetember 1 2016 485\u2013486."},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11051194"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2019.08.002"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2023.02.004"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jepm.2011.11.004"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.1990.51.03.454"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.22233\/9781910443255.18"},{"volume-title":"Effect of Housing on the Health of Pet Rabbits","year":"2020","author":"Winnepenninckx A.","key":"e_1_2_6_16_1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2011.08.019"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.14737\/journal.aavs\/2015\/3.3.183.191"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T03:21:43Z","timestamp":1758165703000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.70041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/vcp.70041"],"URL":"https:\/\/doi.org\/10.1111\/vcp.70041","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"type":"print","value":"0275-6382"},{"type":"electronic","value":"1939-165X"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2023-11-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}