{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T20:04:49Z","timestamp":1785441889532,"version":"3.56.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007709","name":"Michigan State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007709","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n microRNAs (miRNA) have been proposed as biomarkers for pancreatitis in dogs due to their high peak concentrations and experimental correlation with acinar cell injury. However, analytical validation and the effect of interfering substances are unknown.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n The study aimed to (i) develop and analyze the analytical validity of an assay for miRNA\u2010148a and miRNA\u2010375 in serum and (ii) compare miRNA\u2010148a and miRNA\u2010375 between healthy and pancreatitis\u2010affected dogs.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Circulating miRNAs were quantified from serum of healthy (\n n<\/jats:italic>\n \u2009=\u200940) and pancreatitis\u2010affected dogs (\n n<\/jats:italic>\n \u2009=\u200940). Reference intervals were established, and serum miRNAs were compared between groups. Linearity was assessed via dilutional parallelism (observed\/expected ratio, O\/E). Precision was assessed via intra\u2010 and inter\u2010assay coefficients of variation (% CV). Intralipid, bilirubin, and hemoglobin were tested as interferants.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n For miRNA\u2010148a, the RI was <\u200933\u2009000 gene copies and mean O\/E was 100.4%. Mean inter\u2010assay and intra\u2010assay % CV were 1.5% and 1.3% respectively. For miRNA\u2010375, the RI was <\u20094 gene copies and mean O\/E was 100.1%. Mean inter\u2010assay and intra\u2010assay % CV were 3.2% and 0.5% respectively. Hemolysis led to false increases in both assays (\n p\u2009=<\/jats:italic>\n \u20090.003 \u2013 <\u20090.0001). miRNA\u2010148a was reduced in dogs with pancreatitis (mean 192 gene copies) compared with healthy controls (mean 2760 gene copies;\n p<\/jats:italic>\n \u2009<\u20090.0001), but miRNA\u2010375 was not different between groups (\n p<\/jats:italic>\n \u2009=\u20090.94).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The miRNA assays were analytically valid; however, hemolysis, a common interferant in pancreatitis cases, may impact test results. miRNA\u2010148a was suppressed in dogs with pancreatitis. It is unclear whether this represents a cause or a consequence of the disease.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.70067","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T02:49:39Z","timestamp":1763520579000},"page":"440-446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Partial Analytical Validation of\n microRNAs<\/scp>\n \u2010148a and 375 for Detecting Pancreatic Injury in Healthy Dogs and Dogs With Pancreatitis"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2718-6910","authenticated-orcid":false,"given":"Caylen","family":"Erger","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6755-9800","authenticated-orcid":false,"given":"Kyan","family":"Thelen Strong","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0236-2307","authenticated-orcid":false,"given":"Harry","family":"Cridge","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16292"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2004.tb02572.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15693"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12334"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2011.00793.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.17020"},{"key":"e_1_2_7_8_1","first-page":"238","volume-title":"Pharmacokinetics of Pancreatic Lipase in Healthy Dogs","author":"Dossin O.","year":"2011"},{"key":"e_1_2_7_9_1","first-page":"607","article-title":"The 1,2\u2010o\u2010Dilauryl\u2010Rac\u2010Glycero\u20103\u2010Glutaric Acid\u2010(6\u2032\u2010Methylresorufin) Ester (DGGR) Lipase Assay in Cats and Dogs Is Not Specific for Pancreatic Lipase","volume":"49","author":"Lim S. 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