{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:25:49Z","timestamp":1781843149518,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.726177\/2022\u201000"],"award-info":[{"award-number":["88887.726177\/2022\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.936596\/2024\u201000"],"award-info":[{"award-number":["88887.936596\/2024\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Dirofilariosis, a zoonotic disease caused by\n                      <jats:italic>Dirofilaria immitis<\/jats:italic>\n                      , is associated with cardiovascular damage and systemic inflammation in dogs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aimed to present preliminary data on the evaluation of serum haptoglobin (Hp) concentration as a potential biomarker of inflammation in dogs naturally infected with\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>D. immitis<\/jats:italic>\n                      <\/jats:styled-content>\n                      , with and without microfilaremia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Thirty dogs were categorized into three groups: microfilaremic seropositives (G1,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910), amicrofilaremic seropositives (G2,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910), and negative controls (CG,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910). Serum Hp concentrations were measured using a colorimetric assay and analyzed via one\u2010way ANOVA with Tukey's post\u2010test.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Median Hp levels were 10.0\u2009mg\/dL (G1), 9.1\u2009mg\/dL (G2), and 13.7\u2009mg\/dL (CG), with no significant differences among groups. Additionally, no significant correlation was found between microfilarial burden and Hp levels (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.651).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Despite\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>D. immitis<\/jats:italic>\n                      <\/jats:styled-content>\n                      infection, Hp concentration did not provide evidence of an inflammatory response in G1 and G2. While previous studies reported decreased Hp in microfilaremic dogs, our findings did not confirm this trend. The seropositive dogs in this study did not show clinical signs, indicating they had relatively mild infections, which may at least in part explain these results. The small sample size and lack of other acute\u2010phase protein assessments restrict the generalizability of our findings and, thus, this study provides limited information about acute phase response dynamics. Nevertheless, these preliminary results highlight the complexity of Hp behavior in\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>D. immitis<\/jats:italic>\n                      <\/jats:styled-content>\n                      infection and emphasize the need for further research.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vcp.70075","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T05:00:40Z","timestamp":1769749240000},"page":"30-33","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of Haptoglobin Concentrations Between Microfilaremic and Amicrofilaremic Dogs Infected by\n                    <i>Dirofilaria immitis<\/i>"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5065-9725","authenticated-orcid":false,"given":"Marianna L. E.","family":"Chocobar","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science (FMVZ) S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P.","family":"Eckersall","sequence":"additional","affiliation":[{"name":"School of Biodiversity, One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Panarese","sequence":"additional","affiliation":[{"name":"School of Biodiversity, One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Weir","sequence":"additional","affiliation":[{"name":"School of Biodiversity, One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M. S.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science (FMVZ) S\u00e3o Paulo State University (UNESP)  Botucatu SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"American Heartworm Society Canine Guidelines for the Prevention, Diagnosis, and Management of Heartworm (Dirofilaria immitis) Infection in Dogs","author":"AHS\u2014American Heartworm Society","year":"2024"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00012\u201012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20103024.2001.00400.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2003.07.013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.04.009"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939\u2010165x.2005.tb00019.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010017\u20102426\u20108"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.02.036"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.05.016"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12010003"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s005800050020"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580\u2010018\u20102863\u20106"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2022.109759"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1371690"},{"key":"e_1_2_9_16_1","article-title":"Hematological and Biochemical Studies on Filariasis of Dogs","volume":"4","author":"Hashem M.","year":"2007","journal-title":"Journal of Veterinary Medicine"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2019.07.008"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70075","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.70075","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:43:41Z","timestamp":1778906621000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.70075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,29]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/vcp.70075"],"URL":"https:\/\/doi.org\/10.1111\/vcp.70075","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,29]]},"assertion":[{"value":"2025-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}