{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T05:10:50Z","timestamp":1778908250697,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n In teleosts, it is often difficult to differentiate between cutaneous neoplasms originating from peripheral nerves and fibrous tissues, and chromatophoromas, and ultrastructural examination is frequently required in addition to light microscopy for definitive diagnosis.<\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n \n A 10\u2010year\u2010old koi (\n Cyprinus rubrofuscus<\/jats:italic>\n ) had a 3\u2010week history of a 3\u20134\u2009cm, cutaneous, ulcerated, multinodular mass on the dorsum.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Fine needle aspiration revealed spindle cells with abundant, cytoplasmic, variably granular magenta pigment and moderate cellular and nuclear atypia. Biopsy was performed several weeks later and revealed neoplastic spindle cells forming intersecting streams separated by collagenous stroma. Ultrastructural examination showed neoplastic cells had cytoplasmic extensions that were continuous with collagen fibrils, consistent with fibrosarcoma; neoplastic cells lacked pigment and refracting organelles, ruling out chromatophoroma. The nature of the intracytoplasmic pigmented material observed on cytology is uncertain. Neither ultrastructural nor histologic correlates for this cytologically evident material were identified, and differentials for the material are discussed.<\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n In domestic animals, the presence or absence of concurrent inflammation can often be used to differentiate spindle cell neoplasia from fibroplasia on cytology; this feature is less useful in teleosts, however, as sarcomas are often heavily inflamed and chronic granulomas can be composed of a nearly uniform population of spindle cells. Additionally, there is morphologic overlap between poorly pigmented chromatophoromas and variants of soft tissue sarcoma (STS). For these reasons, definitive diagnosis of STS, chromatophoromas, and many other neoplasms in teleosts often requires a combination of light microscopy and transmission electron microscopy.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.70096","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:01:27Z","timestamp":1773000087000},"page":"49-54","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytologic, Histologic, and Ultrastructural Findings of Spontaneous, Cutaneous Fibrosarcoma in a Koi"],"prefix":"10.1111","volume":"55","author":[{"given":"Elise E. B.","family":"LaDouceur","sequence":"first","affiliation":[{"name":"Joint Pathology Center Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrienne E.","family":"Barrett","sequence":"additional","affiliation":[{"name":"Joint Pathology Center Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anibal G.","family":"Armien","sequence":"additional","affiliation":[{"name":"California Animal Health and Food Safety Laboratory System Davis California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Lore","sequence":"additional","affiliation":[{"name":"Bay Animal and Aquatic Hospital St. Petersburg Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4258-4414","authenticated-orcid":false,"given":"Nicole I.","family":"Stacy","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine University of Florida Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2024.1846-1854"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.13008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119380559.ch63"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvex.2006.11.002"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvex.2004.04.012"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985813480529"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14030464"},{"key":"e_1_2_7_10_1","volume-title":"ACVP Annual Meeting. St. Louis, MO","author":"Cudd S.","year":"2019"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12285"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11092621"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.1995.tb00925.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsirep.2021.100029"},{"key":"e_1_2_7_15_1","volume-title":"Surgical Pathology of Tumors of Domestic Animals","author":"Roccabianca P.","year":"1974"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2018.11.011"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19123929"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvex.2016.07.002"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70096","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.70096","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:44:14Z","timestamp":1778906654000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.70096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/vcp.70096"],"URL":"https:\/\/doi.org\/10.1111\/vcp.70096","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}