{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:49:20Z","timestamp":1792612160939,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Pancreatic lipase assays are commonly utilized in the diagnostic approach to suspected pancreatitis in dogs. While a previously available point\u2010of\u2010care assay for the quantification of canine pancreatic lipase immunoreactivity (Vcheck cPL 1.0) performed sub\u2010optimally, the updated version (Vcheck cPL 2.0) lacks independent validation.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To evaluate the analytic validity of the Vcheck cPL 2.0 assay.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Linearity was assessed via dilutional parallelism. Intra\u2010 and inter\u2010assay variability were assessed by calculating coefficients of variation across replicates on the same day and across consecutive days, respectively. Interference studies were performed to assess the effects of bilirubin, hemoglobin, and lipid presence in test samples on assay performance.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Linearity appeared within target parameters at pancreatic lipase concentrations up to ~800 md\/dL (mean O\/E ratio: 0.968\u2009\u00b1\u20090.104) but was unacceptable at pancreatic lipase concentrations >\u20092000\u2009mg\/dL (mean O\/E ratio: 1.432\u2009\u00b1\u20090.178). Intra\u2010assay precision (median coefficient of variation 7.2%) was acceptable; however, inter\u2010assay precision was sub\u2010optimal (coefficient of variation >\u200910%) in 50% of samples analyzed. Variable precision may result in different diagnostic interpretations. Icterus and lipemia did not impact assay results. However, hemolysis, when severe (375\u2013500\u2009mg\/dL), may impact test results (\n p<\/jats:italic>\n \u2009<\u20090.005).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The Vcheck cPL 2.0 shows improved precision compared to previous versions. Linearity was unacceptable at higher pancreatic lipase concentrations and hemolysis may impact Vcheck cPL 2.0 assay results.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.70106","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:29:46Z","timestamp":1777703386000},"page":"217-221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analytical Validation of an Automated Point\u2010of\u2010Care Immunoassay for the Measurement of Canine Pancreatic Lipase Immunoreactivity Concentration (Vcheck\n cPL<\/scp>\n 2.0)"],"prefix":"10.1111","volume":"55","author":[{"given":"Isabel","family":"Mendoza\u2010White","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joerg M.","family":"Steiner","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0236-2307","authenticated-orcid":false,"given":"Harry","family":"Cridge","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine Michigan State University East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16292"},{"issue":"3","key":"e_1_2_7_3_1","first-page":"189","article-title":"A Critical Evaluation of Serum Lipase and Amylase as Diagnostic Tests for Acute Pancreatitis","volume":"2","author":"Jasdanwala S.","year":"2015","journal-title":"Integrative Molecular Medicine"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.22.03.0144"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15039"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1421103"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.24001"},{"key":"e_1_2_7_8_1","unstructured":"E.RozanskiandJ.Wells \u201cValidation of a Point\u2010Of\u2010Care Assay for Serum Canine Pancreatic Lipase and C\u2010Reactive Protein in the Clinical Setting \u201daccessed July 15 2025 www.bionote.com\/_files\/ugd\/934ee6_8a911d84d7794154a9b8a0048d115df5.pdf."},{"key":"e_1_2_7_9_1","unstructured":"J.Steiner J.Liu J.Drexel andC.Chandrashekar \u201cPartial Analytical Validation of a New In\u2010Clinic cPLI Test (VCheck cPL). Presented at: World Small Animal Veterinary Association Congress Proceedings \u201d2018 https:\/\/veterinarypartner.vin.com\/apputil\/project\/defaultadv1.aspx?pid=22915&catid=&id=8896878&meta=&authorid=."},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15763"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.13207"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12796"},{"key":"e_1_2_7_13_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2025"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2010.00251.x"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70106","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.70106","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:35:24Z","timestamp":1792611324000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.70106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,1]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/vcp.70106"],"URL":"https:\/\/doi.org\/10.1111\/vcp.70106","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,1]]},"assertion":[{"value":"2025-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}