{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:51:20Z","timestamp":1792612280870,"version":"4.5.0"},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The quantification of protein levels is an important part of standard cerebrospinal fluid (CSF) analysis. However, delays in processing due to shipment and\/or laboratory opening schedules, combined with the need to preserve sample stability during storage, pose critical challenges to ensuring accurate diagnoses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Our aim was to assess the stability of CSF proteins in canine and feline samples refrigerated and subsequently frozen.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>CSF samples were prospectively collected from dogs and cats. CSF protein levels were measured: immediately after collection; after refrigeration (at 4\u00b0C for 48\u2009h); and after freezing (at \u221220\u00b0C for 6 to 150\u2009days). Linear regression was performed for each species to evaluate the influence of time and preservation on protein levels. The impact of the variation in CSF protein measurements on clinical interpretation was evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Fourteen dogs and four cats with neurological diseases, and five cats euthanized for reasons unrelated to neurological issues were enrolled in the study. Protein measurements were stable over time in dogs (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.334) and cats (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.785), regardless of the preservation method and timing of analysis. The variation between the CSF protein measurements of fresh and stored samples did not change the interpretation of any results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CSF protein levels in dogs and cats remained stable when samples were refrigerated for 48\u2009h prior to being frozen for long\u2010term storage, supporting the feasibility of banking samples for research and laboratory control. Additional studies are necessary to evaluate the stability of specific protein biomarkers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vcp.70131","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T01:46:01Z","timestamp":1785289561000},"page":"136-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stability of Cerebrospinal Fluid Proteins in Dogs and Cats: Effects of Refrigeration and Freezing Over Time"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1213-8907","authenticated-orcid":false,"given":"Luana S.","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Agr\u00e1rias Universidade Federal dos Vales Do Jequitinhonha e Mucuri  Una\u00ed Minas Gerais Brazil"},{"name":"Escola de Veterin\u00e1ria e Zootecnia Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4895-6170","authenticated-orcid":false,"given":"Aude","family":"Castel","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 de Montr\u00e9al  Saint\u2010Hyacinthe Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno B. J.","family":"Torres","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria e Zootecnia Universidade Federal de Goi\u00e1s  Goi\u00e2nia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782006000600021"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12107"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939\u2010165x.2006.tb00090.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr9005876"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2013.03.011"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201073812\u2010y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2005.07.021"},{"key":"e_1_2_9_9_1","first-page":"61","volume-title":"Practical Guide to Canine and Feline Neurology","author":"Dewey C. W.","year":"2016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/vetr.27"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.24.05.0345"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70131","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.70131","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:35:14Z","timestamp":1792611314000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.70131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/vcp.70131"],"URL":"https:\/\/doi.org\/10.1111\/vcp.70131","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}