{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T06:00:40Z","timestamp":1784786440754,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100001458","name":"American Kennel Club Canine Health Foundation","doi-asserted-by":"publisher","award":["02987"],"award-info":[{"award-number":["02987"]}],"id":[{"id":"10.13039\/100001458","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute myeloid leukemia (AML) is difficult to distinguish from lymphoblastic leukemia or lymphoma (LL) on morphologic features alone. Therapeutic approaches for AML versus LL differ, and immediate therapy is often needed due to patient illness and rapid disease progression. Immunophenotyping is required for the distinction of AML from LL but is often not rapidly available. Hence, a routinely available method for distinguishing AML from LL at the time of diagnosis, such as myeloperoxidase (MPX) activity in tumor cells, is desirable.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine whether MPX activity on the ADVIA peroxidase cytogram differentiates AML vs LL, as judged by a lower angle of a line intersecting the large unstained cell (LUC) cluster and the x\u2010axis on the ADVIA 2120 MPX cytogram (MPX LUC angle).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Dogs with suspect acute leukemia were prospectively enrolled, and ADVIA\u2010based hematology, flow cytometry, and cytochemical staining were performed in a multi\u2010institutional study. An observer blinded to sample identity determined the MPX LUC angle with Fiji software.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Forty\u2010four cases met the inclusion criteria and were grouped as AML (\n                      <jats:italic>n<\/jats:italic>\n                      = 30) or LL (\n                      <jats:italic>n<\/jats:italic>\n                      = 14). No significant difference in the mean MPX LUC angle was identified between AML and LL (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.9). MPX cytochemical staining did not correlate with MPX LUC angle (Rsp = 0.004,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&gt;\u00a00.9).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The angle of the LUC population on the ADVIA MPX cytogram cannot be used as a rapid test to differentiate AML from LL.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vcp.70134","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:02:21Z","timestamp":1784782941000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of\n                    <scp>ADVIA<\/scp>\n                    Myeloperoxidase Cytogram\n                    <scp>LUC<\/scp>\n                    Angle Does Not Distinguish Acute Myeloid Leukemia From Acute Lymphoblastic Leukemia or Lymphoblastic Lymphoma in Dogs"],"prefix":"10.1111","author":[{"given":"Mayzie","family":"Miller","sequence":"first","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences University of Tennessee  Knoxville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4543-7313","authenticated-orcid":false,"given":"Nora L.","family":"Springer","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences University of Tennessee  Knoxville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothee","family":"Bienzle","sequence":"additional","affiliation":[{"name":"Department of Pathobiology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8982-2697","authenticated-orcid":false,"given":"Kristina","family":"Meichner","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Stokol","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12377"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/03009858241277982"},{"key":"e_1_2_9_4_1","volume-title":"WHO Classification of Tumours: Haematolymphoid Tumours, Part A","author":"Khoury 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