{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:06:48Z","timestamp":1788948408281,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003454","name":"Guangxi Zhuang Autonomous Region Health Department","doi-asserted-by":"publisher","award":["Z\u2010A20230408"],"award-info":[{"award-number":["Z\u2010A20230408"]}],"id":[{"id":"10.13039\/501100003454","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n In laboratory practice, complete blood count (CBC) analysis is not always immediate, and underfilled or overfilled blood tubes may yield misleading results.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n To assess CBC stability in rats and rabbits over 24\u2009h and determine the effect of under\u2010 or over\u2010filled tubes on alterations in the results, demonstrating which indices remain reliable under these conditions.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Blood was collected into EDTA\u2010K\n 2<\/jats:sub>\n vacuum tubes from rats (tubes: 0.5, 1.0, 1.5, 2.0\u2009mL) and rabbits (tubes: 2.0, 2.5\u2009mL). All tubes were stored at 4\u00b0C and analyzed at 0, 4, 8, and 24\u2009h using a CBC analyzer.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Statistically significant changes (\n p<\/jats:italic>\n \u2009<\u20090.05) were observed: In rats, white blood cell (WBC), neutrophil, lymphocyte, monocyte, red blood cell (RBC), hematocrit (HCT), and hemoglobin (Hb) counts were sensitive to blood sampling volume and decreased with smaller volumes. WBC, neutrophil, RBC, HCT, and platelet (PLT) counts showed no statistically significant change within 24\u2009h. In rabbits, blood sampling volume and storage time independently influenced WBC, neutrophil, lymphocyte, and Hb counts. RBC, HCT, and PLT counts showed no statistically significant change within 8\u2009h.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n WBC, neutrophil, lymphocyte, RBC, HCT, and Hb are volume\u2010sensitive in rats and rabbits, whereas PLT counts are unaffected by volume. Therefore, blood samples with insufficient or excessive fill may still be used for PLT measurement, but results for the other indices are not recommended.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.70140","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:12:31Z","timestamp":1788945151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stability of Complete Blood Count Parameters in Rats and Rabbits: Impact of Blood Sampling Volume and Storage Time on Analytical Reliability"],"prefix":"10.1111","author":[{"given":"Yan\u2010Ni","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Occupational Health and Toxicity Assessment Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Toxicology, School of Public Health Guangxi Medical University Nanning China"},{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Han","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Toxicology, School of Public Health Guangxi Medical University Nanning China"},{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010An","family":"Ling","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Toxicity Assessment Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Ze","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Toxicity Assessment Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010Wen","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Toxicity Assessment Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Occupational Health and Toxicity Assessment Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Aschner","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology Albert Einstein College of Medicine New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5123-3072","authenticated-orcid":false,"given":"Yue\u2010Ming","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Toxicology, School of Public Health Guangxi Medical University Nanning China"},{"name":"Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases Guangxi Medical University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/jat.727","article-title":"A Good Practice Guide to the Administration of Substances and Removal of Blood, Including Routes and Volumes","volume":"21","author":"Diehl K. 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