{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T06:04:51Z","timestamp":1773036291680,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T00:00:00Z","timestamp":1361491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Topical antimicrobial treatment for canine pyoderma is becoming increasingly important, but little is known about the mechanism of action and persistence of activity of antimicrobial shampoos.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the residual antimicrobial activity on canine hairs treated with antimicrobial shampoos.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Forty\u2010two dogs from a research institution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Dogs were treated with six different shampoos and the combination of one shampoo and conditioner containing benzoyl peroxide, chlorhexidine in different concentrations (0.8, 2, 3 and 4%), ethyl lactate and miconazole twice weekly for 2\u00a0weeks. A shampoo vehicle without antimicrobial ingredients was used as the control. Hairs were collected immediately after and 2, 4 and 7\u00a0days after the last shampoo therapy and placed onto an agar plate streaked with<jats:italic>Staphylococcus pseudintermedius<\/jats:italic>. After incubation, the growth inhibition zone around the hair shafts was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The largest zone of inhibition of bacterial growth was seen after shampoos containing 2 and 3% chlorhexidine and the combination of chlorhexidine shampoo and conditioner. The zone of inhibition was smaller with the shampoos containing 0.8 and 4% chlorhexidine. There was no difference between the inhibition zones post\u2010treatment with benzoyl peroxide, ethyl lactate and control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and clinical importance<\/jats:title><jats:p>The efficacy of a shampoo is dependent not only on the concentration of the active ingredients but also on the shampoo formulation. Hair shafts treated with shampoos containing 2 and 3% chlorhexidine and the combination of shampoo and conditioner inhibited bacterial growth significantly and seem suitable to treat canine bacterial pyoderma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12012","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T08:47:13Z","timestamp":1361522833000},"page":"250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Residual antibacterial activity of dog hairs after therapy with antimicrobial shampoos"],"prefix":"10.1111","volume":"24","author":[{"given":"Isabell","family":"Kloos","sequence":"first","affiliation":[{"name":"Centre for Clinical Veterinary Medicine Veterinary Faculty LMU Munich Veterinaerstra\u00dfe 13 D\u201080539 M\u00fcnchen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard 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