{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:05:13Z","timestamp":1774314313420,"version":"3.50.1"},"reference-count":7,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T00:00:00Z","timestamp":1366934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>emodex gatoi<\/jats:italic> causes a pruritic dermatitis in cats. Diagnosis requires the demonstration of mites using superficial skin scrapings or faecal flotation, which can be insensitive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>The goal of this study was to develop a molecular method to diagnose <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> infection in cats and distinguish these mites from <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>emodex cati<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Fifty\u2010three shelter cats, 11 cats from a closed research colony and 12 privately owned cats were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>emodex gatoi<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati<\/jats:italic> were obtained from scrapings of cat skin. The 16<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content> r<jats:styled-content style=\"fixed-case\">RNA DNA<\/jats:styled-content> was amplified by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, sequenced and compared with available <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>emodex<\/jats:italic> sequences. Hair and skin samples were also collected for microscopic examination and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> isolation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequences were obtained from <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati<\/jats:italic>. q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> with <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> specific primers and probe amplified <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> isolated from <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> and not <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati<\/jats:italic>. Conversely, <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati<\/jats:italic> q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> primers and probe amplified <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and not <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic>.<\/jats:p><jats:p>Five of the shelter cats were positive for <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic>. Two of these cats were pruritic, and the other three were in contact with these cats. Only one cat was positive for <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> on skin scraping but was negative for <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> or <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Results from this study show <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati<\/jats:italic> to be distinct species. A novel q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> test for the identification and differentiation of <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0cati<\/jats:italic> was developed. Once optimized, this test could provide a valuable technique for the diagnosis of <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.\u00a0gatoi<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12030","type":"journal-article","created":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T15:26:42Z","timestamp":1366990002000},"page":"367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["A molecular technique for the detection and differentiation of <i><scp>D<\/scp>emodex<\/i> mites on cats"],"prefix":"10.1111","volume":"24","author":[{"given":"Linda A.","family":"Frank","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences University of Tennessee Knoxville TN 37996\u20104544 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Kania","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Tennessee Knoxville TN 37996\u20104544 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karianne","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences University of Tennessee Knoxville TN 37996\u20104544 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupal","family":"Brahmbhatt","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Tennessee Knoxville TN 37996\u20104544 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X12439268"},{"key":"e_1_2_5_3_1","first-page":"671","article-title":"An unusual species of Demodex mite in a cat","volume":"123","author":"Chesney CJ","year":"1988","journal-title":"Vet Rec"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.03.015"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.157.10.290"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-51-40"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2062-0"},{"key":"e_1_2_5_8_1","first-page":"284","volume-title":"Muller & Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12030","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T17:09:15Z","timestamp":1698512955000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,26]]},"references-count":7,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1111\/vde.12030"],"URL":"https:\/\/doi.org\/10.1111\/vde.12030","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,26]]},"assertion":[{"value":"2013-02-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}