{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:40:35Z","timestamp":1780717235558,"version":"3.54.1"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T00:00:00Z","timestamp":1370822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Fluid secretion by sweat glands in response to heat and exercise is underpinned by increases in intracellular calcium. In horses, this is primarily via \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptors, but studies in equine sweat gland cell lines have indicated a possible role for purinergic agonists. Knowledge of equine sweating stimulus\u2013secretion mechanisms in intact glands from healthy animals would allow future comparison to determine whether these mechanisms are affected in equine anhidrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To determine whether purinergic agonists can induce changes in intracellular calcium in intact, freshly isolated equine sweat glands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Eleven healthy thoroughbred horses from the Hong Kong Jockey Club were used in this study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Freshly isolated equine sweat glands were loaded with the calcium\u2010sensitive fluorescent dye fura\u20102\u00a0<jats:styled-content style=\"fixed-case\">AM<\/jats:styled-content>, and changes in intracellular calcium were recorded before, during and after stimulation by purinergic agonists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Purinergic agonists<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">UTP<\/jats:styled-content>generated significant increases in intracellular calcium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>The results show that it is possible to investigate stimulus\u2013secretion coupling mechanisms by fluorescence imaging in equine sweat glands that have been isolated from fresh skin samples. Such isolated glands retain functional \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptors and P2Y purinergic receptors that couple to a calcium\u2010signalling pathway. Using isolated, intact sweat glands therefore offers a very useful model for the further study of secretory processes in equine sweat glands, and using this experimental approach could facilitate a better understanding of how these mechanisms are affected in equine anhidrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12042","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T11:59:14Z","timestamp":1370865554000},"page":"398","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evidence of purinergic neurotransmission in isolated, intact horse sweat glands"],"prefix":"10.1111","volume":"24","author":[{"given":"Douglas L.","family":"Bovell","sequence":"first","affiliation":[{"name":"Department of Biological and Biomedical Sciences Glasgow Caledonian University Cowcaddens Road Glasgow G4 0BA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Riggs","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Hong Kong Jockey Club Shatin New Territories Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Sidlow","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Hong Kong Jockey Club Shatin New Territories Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Troester","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Hong Kong Jockey Club Shatin New Territories Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"MacLaren","sequence":"additional","affiliation":[{"name":"Department of Biological and Biomedical Sciences Glasgow Caledonian University Cowcaddens Road Glasgow G4 0BA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallace","family":"Yip","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The Chinese University of Hong Kong Shatin New Territories Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wing H.","family":"Ko","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The Chinese University of Hong Kong Shatin New Territories Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"41P","article-title":"A physiological explanation of equine anhidrosis","volume":"2","author":"Lovatt Evans C","year":"1956","journal-title":"J Physiol Proceedings"},{"key":"e_1_2_8_3_1","first-page":"1","article-title":"Physiological factors in the condition of \u201cDry Coat\u201d in horses","volume":"69","author":"Lovatt Evans C","year":"1957","journal-title":"Vet Rec"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.2460\/ajvr.1977.38.07.1041","article-title":"Physiologic responses of the horse to a hot arid environment","volume":"38","author":"Honstein RN","year":"1977","journal-title":"Am J Vet Res"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(05)80003-X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2006.00545.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)33164-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4492(82)90118-6"},{"key":"e_1_2_8_9_1","first-page":"R349","article-title":"Predominantly \u03b2\u2010adrenergic control of equine sweating","volume":"246","author":"Bijman J","year":"1984","journal-title":"Am J Physiol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2000.15.S.S63"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.247.1.C10"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1242\/jeb.183.1.279","article-title":"Investigation of stimulus\u2013secretion coupling in equine sweat gland epithelia using cell culture techniques","volume":"183","author":"Wilson SM","year":"1993","journal-title":"J Exp Biol"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1242\/jeb.190.1.239","article-title":"Extracellular ATP can activate autonomic signal transduction pathways in cultured equine sweat gland epithelial cells","volume":"190","author":"Ko W","year":"1994","journal-title":"J Exp Biol"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9629(95)00011-U"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021431"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021746"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1994.sp003779"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111917200"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.92.2.241"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00659.x"},{"key":"e_1_2_8_21_1","first-page":"R44","article-title":"Pharmacologic responsiveness of isolated single eccrine sweat glands","volume":"240","author":"Sato K","year":"1981","journal-title":"Am J Physiol"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1981.241.3.C113"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1242\/jcs.72.1.259","article-title":"Biochemical and ultrastructural studies of human eccrine sweat glands isolated by shearing and maintained for seven days","volume":"72","author":"Lee CM","year":"1984","journal-title":"Cell Sci"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/58.3.545"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"C189","DOI":"10.1152\/ajpcell.1983.245.3.C189","article-title":"Cholinergic potentiation of isoproterenol\u2010induced cyclic AMP level in sweat glands","volume":"245","author":"Sato K","year":"1983","journal-title":"Am J Physiol"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701093"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.056697"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12042","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T12:02:01Z","timestamp":1746014521000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,10]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/vde.12042"],"URL":"https:\/\/doi.org\/10.1111\/vde.12042","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,10]]},"assertion":[{"value":"2013-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}