{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,7]],"date-time":"2024-07-07T16:00:45Z","timestamp":1720368045499},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,10,9]],"date-time":"2013-10-09T00:00:00Z","timestamp":1381276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Mycosis fungoides (<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>) is the most common form of canine epitheliotropic cutaneous lymphoma, which is characterized by the accumulation of neoplastic <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u00a0cells. Given that multifocal skin lesions are commonly seen in <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>, neoplastic lymphocytes may actively migrate into the blood circulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>Cytotoxic <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells with a skin\u2010homing phenotype could be increased in the blood circulation of dogs with <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Ten dogs with <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> and 10 age\u2010matched healthy dogs were included.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The transcription levels of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> were quantified by real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The dogs with MF had lower transcription levels of chemokine receptors associated with skin homing (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4), epitheliotropism (<jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>3), lymph node homing (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>7), a type\u20101 cytokine (<jats:styled-content style=\"fixed-case\">LT<\/jats:styled-content>\u2010\u03b1) and cytotoxic markers (perforin and granzyme\u00a0<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>) in the circulation than healthy control dogs (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>The present results suggest that the number of peripheral cytotoxic <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells with a skin\u2010homing phenotype could be decreased in the peripheral blood of dogs with <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>, which might be due to the sequestration of cytotoxic <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u00a0cells in the lesional skin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12076","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T02:40:10Z","timestamp":1381545610000},"page":"628","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcription profile of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with epitheliotropic cutaneous 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Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Faculty of Applied Biological Sciences Gifu University 1\u20101 Yanagido Gifu 501\u20101193 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Murayama","sequence":"additional","affiliation":[{"name":"ASC Dermatology Service 1\u20103\u20102 Jindaijihigashi Chofu Tokyo 180\u20100012 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Nagata","sequence":"additional","affiliation":[{"name":"ASC Dermatology Service 1\u20103\u20102 Jindaijihigashi Chofu Tokyo 180\u20100012 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Ide","sequence":"additional","affiliation":[{"name":"ASC Dermatology Service 1\u20103\u20102 Jindaijihigashi Chofu Tokyo 180\u20100012 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