{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:38:11Z","timestamp":1788871091284,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T00:00:00Z","timestamp":1401926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"American College of Veterinary Dermatology"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"\n Background<\/jats:title>\n Cutaneous cytology is a valuable tool for diagnosis of canine superficial pyoderma. Current published reproducible techniques are semiquantitative.<\/jats:p>\n <\/jats:sec>\n \n Hypothesis\/Objectives<\/jats:title>\n The aim of this study was to evaluate the reproducibility of a quantitative method for skin surface cytology in dogs with superficial pyoderma.<\/jats:p>\n <\/jats:sec>\n \n Animals<\/jats:title>\n Impression smears were collected from five normal dogs and 20 dogs with clinical and cytological evidence of superficial pyoderma.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Four investigators evaluated 10 oil immersion fields (\n OIF<\/jats:styled-content>\n ) on 25 slides, selecting fields with inflammatory cells, nuclear streaming and or keratinocytes under \u00d710 magnification. Investigators repeated blinded evaluations of all slides at least twice. For each\n OIF<\/jats:styled-content>\n , polymorphonuclear leukocytes (\n PMN<\/jats:styled-content>\n s), intracellular (\n IC<\/jats:styled-content>\n ) cocci, extracellular (\n EC<\/jats:styled-content>\n ) cocci,\n IC<\/jats:styled-content>\n rods,\n EC<\/jats:styled-content>\n rods and yeast were quantified. Nuclear streaming was scored as present or absent. For each parameter, within\u2010reader and between\u2010reader agreements were expressed by the intraclass correlation (\n ICC<\/jats:styled-content>\n ) value (\u22640.20 poor, 0.21\u20130.40 fair, 0.41\u20130.60 moderate, 0.61\u20130.80 good and 0.81\u20131.00 excellent) or kappa statistic (\u03ba).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Reproducible parameters included:\n PMN<\/jats:styled-content>\n s (\n ICC<\/jats:styled-content>\n \u00a0=\u00a00.58), nuclear streaming (\n ICC<\/jats:styled-content>\n \u00a0=\u00a00.68),\n EC<\/jats:styled-content>\n cocci (\n ICC<\/jats:styled-content>\n \u00a0=\u00a00.64) and\n IC<\/jats:styled-content>\n cocci (\n ICC<\/jats:styled-content>\n \u00a0=\u00a00.32). When qualified as present or absent, within\u2010reader \u03ba for\n IC<\/jats:styled-content>\n cocci was 0.71. The method demonstrated 93% sensitivity in identifying dogs with superficial pyoderma and 51% specificity in identifying normal dogs according to established criteria. However, if criteria for normal dogs were limited to the absence of\n PMN<\/jats:styled-content>\n s and\n IC<\/jats:styled-content>\n bacteria, sensitivity of 64% and specificity of 98% were demonstrated.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and clinical importance<\/jats:title>\n \n For several parameters, including\n PMN<\/jats:styled-content>\n s, nuclear streaming,\n EC<\/jats:styled-content>\n cocci and\n IC<\/jats:styled-content>\n cocci, a reproducible, quantitative cytological technique was identified.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vde.12138","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T03:52:55Z","timestamp":1401940375000},"page":"435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Reproducibility of a quantitative cutaneous cytological technique"],"prefix":"10.1111","volume":"25","author":[{"given":"Tyler J.","family":"Udenberg","sequence":"first","affiliation":[{"name":"Animal Dermatology Clinic 4834 Lincoln Boulevard Marina Del Rey CA 90292 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig E.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic 5610 Kearny Mesa Road San Diego CA 92111 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne S.","family":"Rosenkrantz","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic 2965 Edinger Avenue Tustin CA 92780 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudayna M.","family":"Ghubash","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic 4834 Lincoln Boulevard Marina Del Rey CA 90292 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Angus","sequence":"additional","affiliation":[{"name":"Animal Dermatology Clinic 150\u00a0North San Gabriel Boulevard, Suite 400 Pasadena CA 91107 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayak L.","family":"Polissar","sequence":"additional","affiliation":[{"name":"The Mountain\u2010Whisper\u2010Light Statistics 1827 23rd Avenue East Seattle WA 98112 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moni B.","family":"Neradilek","sequence":"additional","affiliation":[{"name":"The Mountain\u2010Whisper\u2010Light Statistics 1827 23rd Avenue East Seattle WA 98112 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctsap.2006.05.004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2003.222.964"},{"key":"e_1_2_6_4_1","first-page":"57","volume-title":"Muller & Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01075.x"},{"key":"e_1_2_6_6_1","first-page":"192","volume-title":"Muller & Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.158.13.437"},{"key":"e_1_2_6_8_1","unstructured":"ColomboS.Quantitative evaluation of cutaneous bacteria in normal dogs and dogs with pyoderma by cytological evaluation. Doctoral Thesis Faculty of Veterinary Medicine Milan Italy 1997."},{"key":"e_1_2_6_9_1","first-page":"243","volume-title":"Muller & Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00721.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.2002.00308.x"},{"key":"e_1_2_6_12_1","first-page":"311","article-title":"Evaluation of four different cytologic evaluation methods in veterinary dermatology","volume":"21","author":"Mueller RS","year":"2010","journal-title":"Vet Dermatol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2011.01020.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2466\/pr0.1966.19.1.3"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016647407161"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.2002.00288.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00113.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00678.x"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12138","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T02:39:02Z","timestamp":1695523142000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,5]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/vde.12138"],"URL":"https:\/\/doi.org\/10.1111\/vde.12138","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,5]]},"assertion":[{"value":"2014-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}