{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T02:11:08Z","timestamp":1692583868154},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T00:00:00Z","timestamp":1410825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A novel bivalent vaccine to protect against myxomatosis and rabbit haemorrhagic disease is commercially available for pet rabbits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To describe the appearance of cutaneous lesions arising in pet rabbits positive for myxoma virus (MV) by RT\u2010PCR evaluation shortly after vaccination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Four pet rabbits presenting with papular, crusting skin lesions ~10\u00a0days after vaccination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Histological evaluation of formalin\u2010fixed skin biopsies obtained from lesional skin (case\u00a01). Real\u2010time polymerase chain reaction (RT\u2010PCR) evaluation of paraffin\u2010embedded tissue from skin biopsies (case\u00a01) and crusts obtained from the lesion surface (cases\u00a02\u20134) for myxoma virus are reported as cycle threshold (<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>) values.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Lesions affecting the ear pinna, dorsal aspect of the nose, vulva and\/or conjunctiva are reported. Histopathological findings included severe ulcerative, necrotizing dermatitis and intralesional cytoplasmic inclusion bodies in myxoma cells. DNA was amplified from all the paraffin\u2010embedded skin biopsies (<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>\u00a0=\u00a034\u201335) and crusts (<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>\u00a0=\u00a020\u201324).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>Although a wild virus challenge cannot be definitively excluded, veterinarians and pet\u2010owners should be aware that cutaneous lesions have been observed after vaccination with this novel vaccine in low numbers of rabbits.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12165","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T12:58:14Z","timestamp":1410872294000},"page":"563","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cutaneous lesions in pet rabbits following subcutaneous administration of a novel bivalent vaccine against myxomatosis and rabbit haemorrhagic disease"],"prefix":"10.1111","volume":"25","author":[{"given":"Paolo","family":"Selleri","sequence":"first","affiliation":[{"name":"Clinica per Animali Esotici Centro Veterinario Specialistico Via Sandro Giovannini 53 00145 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Di Girolamo","sequence":"additional","affiliation":[{"name":"Clinica per Animali Esotici Centro Veterinario Specialistico Via Sandro Giovannini 53 00145 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"V\u00f6gtlin","sequence":"additional","affiliation":[{"name":"Dermatology Service Centro Veterinario Specialistico Via Sandro Giovannini 53 00145 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Fileccia","sequence":"additional","affiliation":[{"name":"Institute for Veterinary Bacteriology National Reference Center for Poultry and Rabbit Diseases (NRGK) University of Zurich Winterthurerstrasse 270 8057 Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Hoop","sequence":"additional","affiliation":[{"name":"Dermatology Service Centro Veterinario Specialistico Via Sandro Giovannini 53 00145 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Bongiovanni","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine University of Teramo Piazza Aldo Moro 45 64100 Teramo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.232.5.695"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0962728600000890"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.e2104"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10344-013-0704-0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.100366"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4160-6621-7.00013-0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711425946"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvex.2013.05.010"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00183-3"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.100321"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12165","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T01:50:25Z","timestamp":1692582625000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,16]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/vde.12165"],"URL":"https:\/\/doi.org\/10.1111\/vde.12165","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,16]]},"assertion":[{"value":"2014-06-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}