{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:30Z","timestamp":1787016030907,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T00:00:00Z","timestamp":1431388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Recent investigations have highlighted the controversial role of Wnt\/\u03b2\u2010catenin pathway activation in human cutaneous melanoma. Survivin has been proposed as a valid prognostic marker for invasive and metastatic melanomas and lymph node melanoma metastasis in human cutaneous melanoma and is a promising therapeutic target.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>Our aim was to investigate the immunohistochemical expression of survivin and \u03b2\u2010catenin in canine cutaneous melanocytic tumours, in order to understand their prognostic significance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty\u2010one melanocytic tumours (10 melanocytomas and 11 melanomas) were investigated by immunohistochemistry using specific anti\u2010survivin and anti\u2010\u03b2\u2010catenin antibodies. A semi\u2010quantitative method was used to analyse the results; \u03b2\u2010catenin immunolabelling in neoplastic cells was evaluated as cytoplasmic, membranous or nuclear. The number of survivin\u2010positive cells was counted within ~1000 neoplastic cells. Results were related to histopathological features, evaluated in haematoxylin\u2010 and eosin\u2010stained slides, and to the clinical data obtained through a telephone survey with referring veterinarians.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Despite a low level of expression in the majority of cases, \u03b2\u2010catenin was found to be correlated strongly with malignant behaviour (\n                      <jats:italic>P\u00a0<\/jats:italic>\n                      &lt;\u00a00.01). An overexpression of nuclear survivin was statistically related to histological features of malignancy, presence of metastasis and death related to melanoma spread (\n                      <jats:italic>P\u00a0<\/jats:italic>\n                      &lt;\u00a00.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>The low nuclear \u03b2\u2010catenin expression, mainly found in metastatic cases, would indicate that \u03b2\u2010catenin activation may have only limited importance in the development or progression of canine cutaneous melanoma. The correlation of nuclear survivin expression with malignancy would indicate that survivin is possibly a useful prognostic marker and therapeutic target in canine melanoma patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.12211","type":"journal-article","created":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T05:18:49Z","timestamp":1431407929000},"page":"270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Canine cutaneous melanocytic tumours: significance of \u03b2\u2010catenin and survivin immunohistochemical expression"],"prefix":"10.1111","volume":"26","author":[{"given":"Laura","family":"Bongiovanni","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine University of Teramo Piazza A. 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