{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T14:23:36Z","timestamp":1784125416962,"version":"3.55.0"},"reference-count":10,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T00:00:00Z","timestamp":1434412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Wipes containing chlorhexidine and azole derivates have been recommended for veterinary use. No study has been published about their activity against <jats:italic>Malassezia pachydermatis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To evaluate the <jats:italic>in\u00a0vivo<\/jats:italic> and <jats:italic>in\u00a0vitro<\/jats:italic> activity of wipes soaked in a chlorhexidine, climbazole and Tris\u2010<jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> solution against <jats:italic>Malassezia pachydermatis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Five research colony shar\u2010pei dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Wipes were applied once daily onto the left axilla, left groin and perianal area (protocol A), and twice daily on the right axilla, right groin and umbilical region (protocol B) for 3\u00a0days. <jats:italic>In vivo<\/jats:italic> activity was evaluated by quantifying <jats:italic>Malassezia<\/jats:italic> colonies through contact plates on the selected body areas before and after wipe application. The activity of the solution in which the wipes were soaked was assessed <jats:italic>in\u00a0vitro<\/jats:italic> by contact tests following the European Standard <jats:styled-content style=\"fixed-case\">UNI EN<\/jats:styled-content> 1275 guidelines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Samples collected after wipe application showed a significant and rapid reduction of <jats:italic>Malassezia<\/jats:italic> yeast <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content>. No significant difference in the <jats:italic>Malassezia<\/jats:italic> reduction was found between protocols A and B. <jats:italic>In vitro<\/jats:italic> assay showed 100% activity against <jats:italic>Malassezia<\/jats:italic> yeasts after a 15\u00a0min contact time with the wipe solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>Wipes containing chlorhexidine, climbazole and Tris\u2010<jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> substantially reduced the <jats:italic>M.\u00a0pachydermatis<\/jats:italic> population on the skin of dogs. The results, although this was an uncontrolled study performed on a small number of dogs, suggest that these wipes may be useful for topical therapy of <jats:italic>Malassezia<\/jats:italic> dermatitis involving the lips, paws, perianal area and skin folds.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12220","type":"journal-article","created":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T11:41:15Z","timestamp":1434454875000},"page":"278","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A pilot study of the efficacy of wipes containing chlorhexidine 0.3%, climbazole 0.5% and Tris\u2010<scp>EDTA<\/scp> to reduce <i>Malassezia pachydermatis<\/i> populations on canine skin"],"prefix":"10.1111","volume":"26","author":[{"given":"Paola","family":"Cavana","sequence":"first","affiliation":[{"name":"Department of Parasitology, Mycology and Dermatology Dynamic Research Group Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort UPE 94704 Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Peano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Yanique","family":"Petit","sequence":"additional","affiliation":[{"name":"Institut de Recherche Clinique d'Alfort Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort UPE 94704 Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Tizzani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Perrot","sequence":"additional","affiliation":[{"name":"Institut de Recherche Clinique d'Alfort Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort UPE 94704 Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Bensignor","sequence":"additional","affiliation":[{"name":"Dermatology Referral Service 6 rue Mare Pav\u00e9e 35510 Rennes\u2010Cesson France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Guillot","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Mycology and Dermatology Dynamic Research Group Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort UPE 94704 Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00721.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01057.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.2003.00334.x"},{"key":"e_1_2_6_5_1","unstructured":"Anon.Chemical disinfectants and antiseptics \u2010 quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics \u2010 test method and requirements (phase 1). EN 1275.Brussels:European Committee For Standardization 2005. Available at:http:\/\/www.en-standard.eu\/csn-en-1275-chemical-disinfectants-and-antiseptics-quantitative-suspension-test-for-the-evaluation-of-basic-fungicidal-or-basic-yeasticidal-activity-of-chemical-disinfectants-and-antiseptics-test-method-and-requirements-phase-1\/. 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