{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:24:44Z","timestamp":1770222284261,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T00:00:00Z","timestamp":1438041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"American College of Veterinary Dermatology","award":["00035869"],"award-info":[{"award-number":["00035869"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Using the US Clinical and Laboratory Standards Institute (CLSI) human tetracycline breakpoints to predict minocycline and doxycycline susceptibility of <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> (SP) isolates from dogs is not appropriate because they are too high to meet pharmacokinetic\/pharmacodynamic data using a standard dose. New breakpoints have been approved for doxycycline and proposed for minocycline. Revised breakpoints are four dilutions lower than tetracycline breakpoints, providing a more conservative standard for classification of isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>The objectives of this study were to measure minimum inhibitory concentrations (MICs) of minocycline and doxycycline of 100 canine meticillin\u2010resistant SP clinical isolates, compare their susceptibilities to minocycline and doxycycline based on current and revised standards, and document their tetracycline resistance genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>E<\/jats:italic>\u2010test strips were used to determine MICs. PCR was used to identify <jats:italic>tet<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using the human tetracycline breakpoint of MIC\u00a0\u2264\u00a04\u00a0\u03bcg\/mL, 76 isolates were susceptible to minocycline and 36 isolates were susceptible to doxycycline. In contrast, using the proposed minocycline breakpoint (MIC\u00a0\u2264\u00a00.25\u00a0\u03bcg\/mL) and approved doxycycline breakpoint (MIC\u00a0\u2264\u00a00.125\u00a0\u03bcg\/mL), 31 isolates were susceptible to both minocycline and doxycycline. Thirty\u2010one isolates carried no <jats:italic>tet<\/jats:italic> genes, two had <jats:italic>tet<\/jats:italic>(K) and 67 had <jats:italic>tet<\/jats:italic>(M).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>Use of the human tetracycline breakpoints misclassified 45 and five of the isolates as susceptible to minocycline and doxycycline, respectively. PCR analysis revealed that 43 and five of the isolates classified as susceptible to minocycline and doxycycline, respectively, possessed the tetracycline resistance gene, <jats:italic>tet<\/jats:italic>(M), known to confer resistance to both drugs. These results underscore the importance of utilizing the proposed minocycline and approved doxycycline canine breakpoints in place of human tetracycline breakpoints.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12227","type":"journal-article","created":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T10:22:54Z","timestamp":1438078974000},"page":"334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of canine\u2010specific minocycline and doxycycline susceptibility breakpoints for meticillin\u2010resistant <i>Staphylococcus pseudintermedius<\/i> isolates from dogs"],"prefix":"10.1111","volume":"26","author":[{"given":"Melanie L.","family":"Hnot","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University 601 Vernon L. Tharp St. Columbus OH 43210 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynette K.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University 601 Vernon L. Tharp St. Columbus OH 43210 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwendolen","family":"Lorch","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University 601 Vernon L. Tharp St. Columbus OH 43210 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark G.","family":"Papich","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences College of Veterinary Medicine North Carolina State University 1060 William Moore Drive Raleigh NC 27607 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paivi J.","family":"Rajala\u2010Schultz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine College of Veterinary Medicine The Ohio State University 601 Vernon L. Tharp St. Columbus OH 43210 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua B.","family":"Daniels","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University 601 Vernon L. Tharp St. Columbus OH 43210 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63413-0"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.06.002"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2010.11.004"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00360-07"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00828.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir147"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2011.01030.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01047.x"},{"key":"e_1_2_5_11_1","unstructured":"RosenthalE.The New York Times website. Officials question the rising cost of generic drugs. Available at:http:\/\/www.nytimes.com\/2014\/10\/08\/business\/officials-question-the-rising-costs-of-generic-drugs.html. Accessed Apr 25 2015."},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.242.12.1674"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.10-0172"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.10.002"},{"key":"e_1_2_5_15_1","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; approved standard\u2013VET01","author":"CLSI","year":"2013"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01498-13"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12130"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01056.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00234-X"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.06.021"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.232-260.2001"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870902100514"},{"key":"e_1_2_5_23_1","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; approved standard\u2013VET01","author":"CLSI","year":"2013"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(00)00130-9"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03445.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2009.0865"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12227","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T09:06:52Z","timestamp":1696151212000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,28]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/vde.12227"],"URL":"https:\/\/doi.org\/10.1111\/vde.12227","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,28]]},"assertion":[{"value":"2015-04-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}