{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T23:11:08Z","timestamp":1761174668583},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education and Science of the Government of Spain","award":["BOE 12022000"],"award-info":[{"award-number":["BOE 12022000"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Involvement of Merkel cells (<jats:styled-content style=\"fixed-case\">MK<\/jats:styled-content>s) in different cutaneous diseases as well as in the growth, differentiation and homeostasis of the skin has been previously documented.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>The aim was to assess the ultrastructural features of <jats:styled-content style=\"fixed-case\">MK<\/jats:styled-content>s in canine skin, including\u00a0morphometrics, highlighting their similarities with and differences from those described for other mammals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Hard palate, nasal planum, lower lip and whisker pad samples were taken from two healthy young dogs destined for academic purposes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ultrathin sections of samples fixed in osmium tetroxide and embedded in Epon\u00a0812 resin were stained with uranyl acetate and lead citrate and examined using a <jats:styled-content style=\"fixed-case\">JEOL JEM<\/jats:styled-content> 2010 transmission electron microscope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ultrastructural characteristics included the following: (i)\u00a0arrangement in clusters in the basal layer of the epidermis, oral mucosa and external follicular root sheath; (ii)\u00a0inconstant link with nerve terminal; (iii)\u00a0oval (10.27\u00a0\u00b1\u00a01.64\u00a0\u03bcm major axis) cell shape with large lobulated nuclei (5.98\u00a0\u00b1\u00a01.16\u00a0\u03bcm major axis); (iv)\u00a0spine\u2010like and thick cytoplasmic processes interdigitating with surrounding keratinocytes; (v)\u00a0presence of desmosomes in the cell body or at the base of spine\u2010like processes attaching to neighbouring keratinocytes; and (vi)\u00a0cytoplasm containing loosely arranged intermediate filaments (10.04\u00a0\u00b1\u00a01.17\u00a0nm) and numerous dense\u2010core granules (100.1\u00a0\u00b1\u00a017.12\u00a0nm) arranged in the basal portion of the cytoplasm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>This study provides the first complete description of the ultrastructural characteristics of <jats:styled-content style=\"fixed-case\">MK<\/jats:styled-content>s in the dog, enhancing our knowledge of the skin structure in this species and providing a basis for future physiological and pathological studies of the role of these cells in normal and damaged canine tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12230","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T22:08:26Z","timestamp":1436911706000},"page":"328","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ultrastructural characterization of normal Merkel cells in the dog"],"prefix":"10.1111","volume":"26","author":[{"given":"Gustavo A.","family":"Ram\u00edrez","sequence":"first","affiliation":[{"name":"Unit 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