{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:31:06Z","timestamp":1762252266125},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T00:00:00Z","timestamp":1442275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"American College of Veterinary Dermatology"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Staphylococcus schleiferi<\/jats:italic> is a known pathogen that can cause canine skin and ear infections. The aim of this study was to determine the molecular epidemiology and antimicrobial susceptibility of clinical veterinary isolates from different geographic regions in the United States.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis<\/jats:title><jats:p>It was hypothesized that <jats:italic>S. schleiferi<\/jats:italic> would maintain genotypic homogeneity across the different geographic regions and that meticillin\u2010resistant (<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>) isolates of <jats:italic>S. schleiferi<\/jats:italic> would predominate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Isolates were identified as <jats:italic>S. schleiferi<\/jats:italic> by a commercial microbiology identification system and confirmed by <jats:italic>nuc<\/jats:italic> gene <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Antibiotic susceptibility data were collected and <jats:styled-content style=\"fixed-case\">PBP<\/jats:styled-content>2a latex agglutination testing was performed on <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> isolates. Pulsed\u2010field gel electrophoresis (<jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>) was performed and clonal clusters were identified with a Dice coefficient similarity of &gt;80%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 116 isolates from the Mid\u2010Atlantic region and 101 from across the United States. Of these 217 isolates, 209 (96%) were obtained from cutaneous sites. Of the Mid\u2010Atlantic isolates, 62% (72 of 116) were <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> and 16% (18 of 116) were multidrug\u2010resistant (<jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>). Of the isolates from the other geographic regions, 73% (74 of 101) were <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> and 24% (24 of 101) were <jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>. All <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> isolates were positive by <jats:styled-content style=\"fixed-case\">PBP<\/jats:styled-content>2a latex agglutination. <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> identified 155 individual pulsed\u2010field profiles and three major pulsed\u2010field types (<jats:styled-content style=\"fixed-case\">PFT<\/jats:styled-content>) that contained 61% (133 of 217) of the isolates. These pulsed\u2010field types were geographically heterogeneous.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrates the dissemination of successful <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> pulsed\u2010field types of <jats:italic>S. schleiferi<\/jats:italic> across the United States.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12254","type":"journal-article","created":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T10:38:16Z","timestamp":1442313496000},"page":"406","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Genotypic relatedness and antimicrobial resistance of <i>Staphylococcus schleiferi<\/i> in clinical samples from dogs in different geographic regions of the United States"],"prefix":"10.1111","volume":"26","author":[{"given":"Darcie A.","family":"Kunder","sequence":"first","affiliation":[{"name":"Department of Clinical Studies \u2013 Philadelphia School of Veterinary Medicine University of Pennsylvania 3900 Delancey Street Philadelphia PA 19104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine L.","family":"Cain","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies \u2013 Philadelphia School of Veterinary Medicine University of Pennsylvania 3900 Delancey Street Philadelphia PA 19104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"O'Shea","sequence":"additional","affiliation":[{"name":"Department of Pathobiology \u2013 Philadelphia School of Veterinary Medicine University of Pennsylvania 3900 Delancey Street Philadelphia PA 19104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen D.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies \u2013 Philadelphia School of Veterinary Medicine University of Pennsylvania 3900 Delancey Street Philadelphia PA 19104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley C.","family":"Rankin","sequence":"additional","affiliation":[{"name":"Department of Pathobiology \u2013 Philadelphia School of Veterinary Medicine University of Pennsylvania 3900 Delancey Street Philadelphia PA 19104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,15]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"184","volume-title":"Muller and Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2005.227.928"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.08.022"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/16.4.589"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/24.5.1014"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.05.027"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.5301\/ejo.5000254"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.72.1.96"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.3389\/fmicb.2013.00123","article-title":"Two coagulase\u2010negative staphylococci emerging as potential zoonotic pathogens: wolves in sheep's clothing?","volume":"4","author":"Davis MF","year":"2013","journal-title":"Front Microbiol"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0436-0"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2004.65.1265"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01336-06"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.239.12.1566"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2003.222.451"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.230.2.221"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2006.00536.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1086\/646235","article-title":"Treatment of infection and colonization caused by methicillin\u2010resistant Staphylococcus aureus","volume":"12","author":"Chambers HF","year":"1991","journal-title":"Infect Control Hosp Epidemiol"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00635.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01047.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.1.117"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.8.1337"},{"key":"e_1_2_5_24_1","volume-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals; Informational Supplement","author":"Clinical Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_5_25_1","volume-title":"Performance Standards for Antimicrobial Suceptibility Testing; Twenty\u2010Third Informational Supplement","author":"Clinical Laboratory Standards Institute","year":"2013"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3946-3951.2001"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01232-09"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5113-5120.2003"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.01.002"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.02.002"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01035.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00868-06"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.06.029"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq078"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12254","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T08:52:55Z","timestamp":1696150375000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,15]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/vde.12254"],"URL":"https:\/\/doi.org\/10.1111\/vde.12254","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,15]]},"assertion":[{"value":"2015-06-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}