{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:36:07Z","timestamp":1761896167001},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T00:00:00Z","timestamp":1442361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007709","name":"Michigan State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007709","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Hereditary equine regional dermal asthenia (<jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>) is a genetic disorder of collagen resulting in fragile, hyper\u2010extensible skin and ulcerative lesions. The predominance of skin lesions have been shown to occur on the dorsum of <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses. While this has been postulated to be due to increased exposure to sunlight of these areas, the precise pathological mechanism which causes this to occur is unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/objectives<\/jats:title><jats:p>We hypothesized that an increase in collagenase activity, that has been associated with the exposure of dermal fibroblasts to sunlight, will significantly degrade the material properties of skin from <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses when compared to unaffected controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Six unaffected and seven <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses, all euthanized for other reasons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Full\u2010thickness skin samples from similar locations on each horse were collected and cut into uniform strips and their material properties (tensile modulus) determined by mechanical testing before (<jats:italic>n<\/jats:italic> = 12 samples\/horse) or after (<jats:italic>n<\/jats:italic> = 12 samples\/horse) incubation in bacterial collagenase at 37\u00b0C for 6 h. The change in modulus following treatment was then compared between <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected and unaffected horses using a Student's <jats:italic>t<\/jats:italic>\u2010test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The modulus of skin from <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses decreased significantly more than that from unaffected horses following collagenase treatment (54 \u00b1 7% versus 30 \u00b1 16%, <jats:italic>P<\/jats:italic> = 0.004).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>The significant decrease in the modulus of skin from <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses following collagenase exposure suggests that their altered collagen microarchitecture is more susceptible to enzymatic degradation and may explain the localization of skin lesions in <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses to those areas of the body most exposed to sunlight. These findings appear to support the previously reported benefits of sunlight restriction in <jats:styled-content style=\"fixed-case\">HERDA<\/jats:styled-content>\u2010affected horses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12256","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T06:25:06Z","timestamp":1442384706000},"page":"476","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increased susceptibility of skin from <scp>HERDA<\/scp> (Hereditary Equine Regional Dermal Asthenia)\u2010affected horses to bacterial collagenase degradation: a potential contributing factor to the clinical signs of <scp>HERDA<\/scp>"],"prefix":"10.1111","volume":"26","author":[{"given":"Ann","family":"Rashmir\u2010Raven","sequence":"first","affiliation":[{"name":"Department of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University 784 Wilson Road G\u2010387 East 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Orthopaedic Research Michigan State University 784 Wilson Road G\u2010387 East Lansing MI 48824 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.333336"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2007.03.009"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.234.1.120"},{"key":"e_1_2_6_5_1","first-page":"827","article-title":"Clinical snapshot, Ehlers\u2010Danlos like syndrome in a quarter horse gelding","volume":"23","author":"Graves MN","year":"2001","journal-title":"Compend Cont Educ Pract Vet"},{"key":"e_1_2_6_6_1","first-page":"350","article-title":"Hyperelastosis in 2 horses","volume":"2","author":"Lerner DJ","year":"1978","journal-title":"J Equine Med Surg"},{"key":"e_1_2_6_7_1","unstructured":"2004 AM Rashmir\u2010Raven NJ Winand RW Read Equine hyperelastosis 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