{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:43:22Z","timestamp":1784641402365,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is associated with otitis and pyoderma in dogs and is frequently resistant to several antimicrobial drugs. Resistance genes can be carried by integrons with quinolone resistance mainly due to mutations in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> topoisomerases <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the antimicrobial susceptibility, integron carriage, and <jats:italic>gyrA<\/jats:italic> and <jats:italic>gyrB<\/jats:italic> mutations in <jats:italic>P. aeruginosa<\/jats:italic> isolates from canine otitis and pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>One hundred and four <jats:italic>P. aeruginosa<\/jats:italic> strains isolated from dogs with otitis externa (<jats:italic>n<\/jats:italic> = 93) and pyoderma (<jats:italic>n<\/jats:italic> = 11).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Antimicrobial susceptibility against 16 antibacterial agents was evaluated through agar diffusion tests. Integron carriage, class and <jats:italic>gyrA<\/jats:italic> and <jats:italic>gyrB<\/jats:italic> mutations were analysed by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, restriction fragment length polymorphism (<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and genetic sequencing assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Isolates were mostly resistant to enrofloxacin (72.2%) and ticarcillin (59.7%). Lower resistance to ciprofloxacin (7.7%), tobramycin (3.8%) and polymixin B (0.0%) was detected. Ten (9.6%) multidrug\u2010resistant (<jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>) strains were detected. Eight (7.7%) strains carried class 1 integrons and this was associated with <jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content> (three isolates, <jats:italic>P<\/jats:italic> \u2264 0.05). Five of the integron\u2010carrying strains exhibited aminoglycoside resistance genes. Mutations of <jats:italic>gyrA<\/jats:italic> and <jats:italic>gyrB<\/jats:italic> were observed in 10 isolates, seven of them resistant to all fluoroquinolones tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>Enrofloxacin and ticarcilin resistance was widespread in <jats:italic>P. aeruginosa<\/jats:italic> isolated from dogs in Brazil. <jats:italic>Pseudomonas aeruginosa<\/jats:italic> carrying integrons may present a significant challenge for treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12290","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T08:44:38Z","timestamp":1454316278000},"page":"113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Antimicrobial resistance, integron carriage, and <i>gyrA<\/i> and <i>gyrB<\/i> mutations in <i>Pseudomonas aeruginosa<\/i> isolated from dogs with otitis externa and pyoderma in Brazil"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4638-0793","authenticated-orcid":false,"given":"Lavicie R.","family":"Arais","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology Federal Fluminense University (UFF) Rua Hernani Mello, 101 Niter\u00f3i 24210130 RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 V.","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Federal Fluminense University (UFF) Rua Hernani Mello, 101 Niter\u00f3i 24210130 RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane A.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Federal Fluminense University (UFF) Rua Hernani Mello, 101 Niter\u00f3i 24210130 RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aloysio M. 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