{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:59:57Z","timestamp":1773035997861,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,7,3]],"date-time":"2016-07-03T00:00:00Z","timestamp":1467504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Topical antimicrobial therapy is increasingly important in the treatment of canine pyoderma as the incidence of multidrug resistance has risen. However, little information is reported on the persistence of activity of topical antimicrobial products.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the residual antibacterial activity of canine hairs treated with antimicrobial sprays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Twelve privately owned dogs with no history of dermatological disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Dogs were treated once with four different spray products [(A) 1% chlorhexidine digluconate, (B) 2% miconazole nitrate, 2% chlorhexidine gluconate, tromethamine <jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content>\/disodium <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> (Triz<jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content>), (C) 3% chlorhexidine gluconate, phytosphingosine salicyloyl and (D) 4% chlorhexidine gluconate, Triz<jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content>] in separate 5\u00a0\u00d7\u00a05\u00a0cm sections on the trunk. Hairs were collected via shaving before, one hour after and 2, 4, 7 and 10\u00a0days after treatment. Hairs were incubated on agar plates streaked with <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> for 24\u00a0h and the bacterial growth inhibition zone around the hairs was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were significant overall treatment and day (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) differences in inhibition zones. The largest zones of inhibition were from hairs treated with spray B, followed by sprays D, C and A, respectively. All sprays demonstrated residual antimicrobial activity for the ten days evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical significance<\/jats:title><jats:p>Results suggest that the efficacy of an antimicrobial spray is dependent on both the concentration and combination of active ingredients. Several ingredient profiles appear to effectively inhibit <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> growth for at least 10\u00a0days, which may be beneficial in the treatment of canine pyoderma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12318","type":"journal-article","created":{"date-parts":[[2016,7,4]],"date-time":"2016-07-04T02:57:55Z","timestamp":1467601075000},"page":"261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Residual antibacterial activity of canine hair treated with topical antimicrobial sprays against <i>Staphylococcus pseudintermedius in\u00a0vitro<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"Mollie L.","family":"Mesman","sequence":"first","affiliation":[{"name":"Animal Dermatology Clinic 4834 Lincoln Boulevard Marina del Rey CA 90293 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison 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