{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:12:16Z","timestamp":1791839536370,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T00:00:00Z","timestamp":1471910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100003516","name":"Winn Feline Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100003516","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Next generation sequencing (<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>) studies have demonstrated a diverse skin\u2010associated microbiota and microbial dysbiosis associated with atopic dermatitis in people and in dogs. The skin of cats has yet to be investigated using<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>We hypothesized that the fungal microbiota of healthy feline skin would be similar to that of dogs, with a predominance of environmental fungi, and that fungal dysbiosis would be present on the skin of allergic cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Eleven healthy cats and nine cats diagnosed with one or more cutaneous hypersensitivity disorders, including flea bite, food\u2010induced and nonflea nonfood\u2010induced hypersensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Healthy cats were sampled at twelve body sites and allergic cats at six sites.<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>was isolated and Illumina sequencing was performed targeting the internal transcribed spacer region of fungi. Sequences were processed using the bioinformatics software<jats:styled-content style=\"fixed-case\">QIIME<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The most abundant fungal sequences from the skin of all cats were classified as<jats:italic>Cladosporium<\/jats:italic>and<jats:italic>Alternaria<\/jats:italic>. The mucosal sites, including nostril, conjunctiva and reproductive tracts, had the fewest number of fungi, whereas the pre\u2010aural space had the most. Allergic feline skin had significantly greater amounts of Agaricomycetes and Sordariomycetes, and significantly less<jats:italic>Epicoccum<\/jats:italic>compared to healthy feline skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The skin of healthy cats appears to have a more diverse fungal microbiota compared to previous studies, and a fungal dysbiosis is noted in the skin of allergic cats. Future studies assessing the temporal stability of the skin microbiota in cats will be useful in determining whether the microbiota sequenced using<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>are colonizers or transient microbes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12373","type":"journal-article","created":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T02:12:43Z","timestamp":1472004763000},"page":"71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Characterization of the cutaneous mycobiota in healthy and allergic cats using next generation sequencing"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5189-2702","authenticated-orcid":false,"given":"Courtney","family":"Meason\u2010Smith","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology College of Veterinary Medicine Texas A&amp;M University 4467 TAMU College 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