{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:01:55Z","timestamp":1783728115971,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,5]],"date-time":"2016-09-05T00:00:00Z","timestamp":1473033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"LDCA (France)"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Bacterial pyoderma is a frequent presentation in dogs. Despite the widespread availability of effective systemic and topical antimicrobial products, good clinical practice currently recommends avoidance of long\u2010term use to mitigate the development of bacterial resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To evaluate the speed of resolution of clinical signs of bacterial pyoderma in dogs treated with a systemic antimicrobial agent with or without the use of an adjunctive spray with antimicrobial properties.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Twelve dogs with superficial bacterial pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this controlled and blinded study, all dogs were treated with oral cefalexin and a topical spray (PYOClean Spray) for 4 weeks. The spray was applied to one half of each dog's body, whereas a placebo spray was applied to the other half.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twelve dogs completed the study. Mean clinical scores were significantly reduced on spray\u2010treated sites, for test product and placebo (respectively), by 47% and 34% at Week 1, 83% and 60% at Week 2, 95% and 82% at Week 3, and 100% and 96% at Week 4. Fifty percent of treated sites were considered clinically and cytologically cured at Week 2, 83% at Week 3, and 100% at Week 4 compared to 8%, 50% and 83% for the placebo sites, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>These results demonstrate that use of a topical spray which contains plant\u2010derived essential oils and fatty acids, and compounds with antimicrobial properties (Manuka oil and N\u2010acetyl cysteine) may help to speed resolution of pyoderma and may allow for shorter antimicrobial treatment time.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12374","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T04:06:41Z","timestamp":1473134801000},"page":"464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A split\u2010body, randomized, blinded study to evaluate the efficacy of a topical spray composed of essential oils and essential fatty acids from plant extracts with antimicrobial properties"],"prefix":"10.1111","volume":"27","author":[{"given":"Emmanuel","family":"Bensignor","sequence":"first","affiliation":[{"name":"Dermatology Referral Service Clinique V\u00e9t\u00e9rinaire 6 rue mare pav\u00e9e 35510 Rennes\u2010Cesson France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Fabri\u00e8s","sequence":"additional","affiliation":[{"name":"Laboratoire de Dermo\u2010Cosm\u00e9tique Animale (LDCA) ZA Le Causse 81100 Castres France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Bailleux","sequence":"additional","affiliation":[{"name":"Laboratoire de Dermo\u2010Cosm\u00e9tique Animale (LDCA) ZA Le Causse 81100 Castres France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.158.16.533"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.101069"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12118"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2011.01165.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01057.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01095.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.144.19.536"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.2736\/jjvd.12.1"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2010.00898.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.d4400"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.100643"},{"key":"e_1_2_5_13_1","first-page":"449","volume":"22","author":"Murayama N","year":"2011","journal-title":"Dose assessment of 2% chlorhexidine acetate for canine superficial pyoderma. 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