{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:47:17Z","timestamp":1761648437611},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:00:00Z","timestamp":1493769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Australian and New Zealand College of Veterinary Scientists and Luoda Pharma"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Topical antimicrobial preparations are of utmost importance in treating suspected and confirmed meticillin\u2010resistant <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> (<jats:styled-content style=\"fixed-case\">MRSP<\/jats:styled-content>) infections due to the increasing incidence of widespread resistance to systemic antimicrobials. Lasalocid is active against <jats:styled-content style=\"fixed-case\">MRSP <\/jats:styled-content><jats:italic>in vitro<\/jats:italic> and this may become an important topical antimicrobial for the treatment of canine pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To determine effects of various formulation types on penetration and retention of lasalocid applied to canine skin <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Normal canine skin was collected from the thorax of five dogs that had been euthanized on the basis of health and\/or intractable behavioural issues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Solution, lotion and ointment containing 2% lasalocid were applied to <jats:italic>ex vivo<\/jats:italic> canine skin. Transdermal penetration was assessed for a 24 h period and retention of lasalocid was assessed at the conclusion of the study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The solution had significantly higher skin retention of lasalocid and proportion of applied dose retained in skin than lotion and ointment (Tukey\u2013Kramer Honest Significant Difference test, <jats:italic>P<\/jats:italic> &lt; 0.01). Lasalocid could not be detected in the receptor fluid of any Franz cell at any time point.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>Lasalocid was not identified in the receptor fluid of any sample, indicating that systemic absorption of the active ingredient <jats:italic>in vivo<\/jats:italic> is unlikely. Lasalocid may be useful in the treatment of MRSP infections if <jats:italic>in vivo<\/jats:italic> studies support safety and efficacy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12379","type":"journal-article","created":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T02:02:41Z","timestamp":1493863361000},"page":"342","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i> comparison of the dermal penetration of three different topical formulations containing lasalocid"],"prefix":"10.1111","volume":"28","author":[{"given":"Evie C.","family":"Knight","sequence":"first","affiliation":[{"name":"Dermatology for Animals 263 Appleby Road Stafford Queensland 4053 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darren J.","family":"Trott","sequence":"additional","affiliation":[{"name":"Australian Centre for Antimicrobial Resistance Ecology School of Animal and Veterinary Sciences The University of Adelaide Roseworthy Campus Mudla Wirra Rd Roseworthy South Australia 5371 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen W.","family":"Page","sequence":"additional","affiliation":[{"name":"Luoda Pharma 1\/304\u2010318 The Kingsway Caringbah New South Wales 2229 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay","family":"Garg","sequence":"additional","affiliation":[{"name":"Centre for Pharmaceutical Innovation and Development School of Pharmacy and Medical Science University of South Australia City East Campus Playford building Frome Rd Adelaide South Australia 5000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Centre for Pharmaceutical Innovation and Development School of Pharmacy and Medical Science University of South Australia City East Campus Playford building Frome Rd Adelaide South Australia 5000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunmei","family":"Song","sequence":"additional","affiliation":[{"name":"Centre for Pharmaceutical Innovation and Development School of Pharmacy and Medical Science University of South Australia City East Campus Playford building Frome Rd Adelaide South Australia 5000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esmaeil","family":"Ebrahimie","sequence":"additional","affiliation":[{"name":"Australian Centre for Antimicrobial Resistance Ecology Department of Genetics &amp; Evolution School of Biological Sciences The University of Adelaide Adelaide South Australia 5005 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul C.","family":"Mills","sequence":"additional","affiliation":[{"name":"School of Veterinary Science University of Queensland Gatton Queensland 4343 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Shipstone","sequence":"additional","affiliation":[{"name":"Dermatology for Animals 263 Appleby Road Stafford Queensland 4053 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.076117-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063871002200617"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.10.023"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq203"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.05.045"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2011.01398.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.09.021"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.01.027"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-2994-2-31"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153624"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00048"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12118"},{"key":"e_1_2_6_14_1","unstructured":"HollowayS TrottDJ ShipstoneMAet al.AIDAP: Antibiotic Prescribing Detailed Guidelines 2013 p46 48. 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