{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:59:30Z","timestamp":1761663570741,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Zenoaq Corporation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Atopic dogs with hypersensitivity to <jats:italic>Dermatophagoides farinae (<\/jats:italic>Df) have IgE recognizing high molecular weight (<jats:styled-content style=\"fixed-case\">MW<\/jats:styled-content>) allergens more often than the low <jats:styled-content style=\"fixed-case\">MW<\/jats:styled-content> Der f 1 and 2. A new high <jats:styled-content style=\"fixed-case\">MW<\/jats:styled-content> Df allergen, Zen\u20101, has been identified recently.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine the IgE reactivity of American and European Df\u2010hypersensitive dogs to Zen\u20101, Der f 1 and Der f 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We tested sera from 33 Df\u2010reactive dogs from the <jats:styled-content style=\"fixed-case\">USA<\/jats:styled-content>, 29 from Europe and 15 experimentally sensitized to Df, by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> against crude Df, Der f 1, Der f 2 and Zen\u20101. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> inhibition was performed with sera reactive to Zen\u20101. Intradermal testing (<jats:styled-content style=\"fixed-case\">IDT<\/jats:styled-content>) was also done with the same allergens in 25 other American atopic dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Altogether, IgE seropositivity to Zen\u20101 was more prevalent (86%) than that to Der f 1 (17%) or Der f 2 (19%). The IgE reactivity to Zen\u20101 was correlated to that against crude Df; this allergen alone inhibited a high percentage (median: 50%; range: 22\u201384%) of the binding to the crude mite extract. The seropositivity to low <jats:styled-content style=\"fixed-case\">MW<\/jats:styled-content> allergens was highest in experimentally sensitized dogs. Serum IgE recognition of Der f 1 was low in dogs with <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>; that to Der f 2 was significantly lower in American dogs (6%) than in European ones (28%). A high prevalence of positive immediate <jats:styled-content style=\"fixed-case\">IDT<\/jats:styled-content> reactions to Zen\u20101 confirmed the likely relevance of serological results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>This study establishes Zen\u20101 as a major allergen in atopic dogs sensitized to Df.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12393","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T07:40:23Z","timestamp":1478504423000},"page":"177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The novel high molecular weight <i>Dermatophagoides farinae<\/i> protein Zen\u20101 is a major allergen in North American and European mite allergic dogs with atopic dermatitis"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1399-0034","authenticated-orcid":false,"given":"Thierry","family":"Olivry","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine NC State University 1060 William Moore Drive Raleigh NC 27607 USA"},{"name":"Comparative Medicine Institute NC State University Raleigh NC 27606 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley M.","family":"Dunston","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine NC State University 1060 William Moore Drive Raleigh NC 27607 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Favrot","sequence":"additional","affiliation":[{"name":"Clinic for Small Animal Internal Medicine Dermatology Department Vetsuisse Faculty University of Z\u00fcrich Winterthurerstrasse 260 CH\u20108057 Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal","family":"Pr\u00e9laud","sequence":"additional","affiliation":[{"name":"Clinique V\u00e9t\u00e9rinaire Advetia 5 Rue Dubrunfaut F\u201075012 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiroh","family":"Tsukui","sequence":"additional","affiliation":[{"name":"Animal Life Science Laboratories Nippon Zenyaku Kogyo Corporation (Zenoaq) 1\u20101 Tairanoue, Sasagawa, Asaka\u2010machi Koriyama Fukushima 963\u20100196 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