{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:46:52Z","timestamp":1761648412862,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nippon Zenyaku Kogyo (Zenoaq)"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"Background<\/jats:title>Allergen immunotherapy is currently the only intervention proposed to specifically prevent clinical flares after allergen challenges. The low molecular weight Der f 2 (Df2) is a major allergen in Japanese dogs sensitized to Dermatophagoides farinae<\/jats:italic> house dust mites.<\/jats:p><\/jats:sec>Objectives<\/jats:title>Pilot, blinded, placebo\u2010controlled experiment testing the efficacy of subcutaneous immunotherapy (SCIT<\/jats:styled-content>) with high doses of recombinant Df2 conjugated to the maltotriose pullulan (rD<\/jats:styled-content>f2\u2010P).<\/jats:p><\/jats:sec>Methods<\/jats:title>Eight Maltese beagle atopic dogs were sensitized to rD<\/jats:styled-content>f2 then randomized to SCIT<\/jats:styled-content> with rD<\/jats:styled-content>f2\u2010P (six dogs) or placebo (two). The immunotherapy consisted of six weekly injections of increasing doses (0.1\u201310.0 \u03bcg) of rD<\/jats:styled-content>f2\u2010P followed by four monthly injections of 10 \u03bcg of this allergen. Epicutaneous rD<\/jats:styled-content>f2 challenges, rD<\/jats:styled-content>f2\u2010specific IgE serology and intradermal reactivity, as well as serum cytokine level measurements, were performed throughout the study.<\/jats:p><\/jats:sec>Results<\/jats:title>Subcutaneous injections of placebo did not alter the cutaneous reactivity after rD<\/jats:styled-content>f2 challenge, while that of the dogs treated with rD<\/jats:styled-content>f2\u2010P SCIT<\/jats:styled-content> disappeared in five of six dogs (83%) and was reduced in one of six (17%). During SCIT<\/jats:styled-content> maintenance, skin lesion scores were significantly lower in dogs receiving SCIT<\/jats:styled-content> compared to those treated with placebo. This clinical improvement was accompanied by a concurrent, yet not significant, decrease in rD<\/jats:styled-content>f2\u2010specific IgE serology and immediate intradermal reactivity. Cytokine serum levels were inconclusive. There were no adverse events seen with rD<\/jats:styled-content>f2\u2010P SCIT.<\/jats:p><\/jats:sec>Conclusions and clinical importance<\/jats:title>The new mono\u2010allergen SCIT<\/jats:styled-content> appears safe and effective for reducing skin lesions after allergen challenges; it deserves further testing in dogs with spontaneous atopic dermatitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12395","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T07:41:44Z","timestamp":1478504504000},"page":"183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Proof of concept of the preventive efficacy of high\u2010dose recombinant mono\u2010allergen immunotherapy in atopic dogs sensitized to the Dermatophagoides farinae<\/i> allergen Der f 2"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1399-0034","authenticated-orcid":false,"given":"Thierry","family":"Olivry","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine NC State University 1060 William Moore Drive Raleigh NC 27607 USA"},{"name":"Comparative Medicine Institute NC State University Raleigh NC 27606 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy S.","family":"Paps","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine NC State University 1060 William Moore Drive Raleigh NC 27607 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley M.","family":"Dunston","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine NC State University 1060 William Moore Drive Raleigh NC 27607 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70271-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.12.1300"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.64.673"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.02949.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2010.00938.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00697.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(97)00119-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00348-8"},{"key":"e_1_2_6_10_1","first-page":"60","volume-title":"Advances in veterinary dermatology","author":"Mueller RS","year":"2005"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(93)90190-Q"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1997.tb00971.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201033489685"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425912447129"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.02.013"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10183"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.02.008"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12284"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2010.00890.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(90)90180-T"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000236536"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12395","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12395","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T17:42:19Z","timestamp":1696614139000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,7]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1111\/vde.12395"],"URL":"https:\/\/doi.org\/10.1111\/vde.12395","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"type":"print","value":"0959-4493"},{"type":"electronic","value":"1365-3164"}],"subject":[],"published":{"date-parts":[[2016,11,7]]},"assertion":[{"value":"2016-09-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}