{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T02:07:46Z","timestamp":1769825266027,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,29]],"date-time":"2017-01-29T00:00:00Z","timestamp":1485648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Feline sporotrichosis is common in Malaysia. Thermosensitivity and effects of azole treatment on fungal susceptibility are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate thermotolerance and antifungal susceptibility of feline Malaysian <jats:italic>Sporothrix<\/jats:italic> isolates<jats:italic>,<\/jats:italic> compare microdilution (<jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>) and E\u2010test results, and investigate changes in susceptibility during azole therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Sporothrix schenckii sensu stricto<\/jats:italic> was isolated from 44 cats. Thermotolerance was determined via culture at 37\u00b0C for 7\u00a0days. Susceptibility to itraconazole (<jats:styled-content style=\"fixed-case\">ITZ<\/jats:styled-content>), ketoconazole (<jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content>) and terbinafine (<jats:styled-content style=\"fixed-case\">TRB<\/jats:styled-content>) was assessed in 40 isolates by <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>; to amphotericin B (<jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ITZ<\/jats:styled-content>, fluconazole (FLC) and posaconazole (POS) by E\u2010test. Results were statistically compared by Pearson's Product Moment. In eight ketoconazole treated cats, susceptibility testing to itraconazole and ketoconazole was repeated every two\u00a0months for six\u00a0months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thermotolerance was observed in 36 of 44 (82%) isolates. Assuming that isolates growing at antifungal concentrations \u22654\u00a0mg\/mL were resistant, all were resistant on E\u2010test to <jats:styled-content style=\"fixed-case\">FLC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AMB<\/jats:styled-content>, 11 (28%) to <jats:styled-content style=\"fixed-case\">POS<\/jats:styled-content>, 6 (15%) to <jats:styled-content style=\"fixed-case\">ITZ<\/jats:styled-content> and 1 (3%) to <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content>. On <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>, 27 of 40 (68%) were resistant to <jats:styled-content style=\"fixed-case\">TRB<\/jats:styled-content>, 2 (5%) to <jats:styled-content style=\"fixed-case\">ITZ<\/jats:styled-content> and 3 (8%) to <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content>. There was no correlation between E\u2010test and <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content> results (<jats:styled-content style=\"fixed-case\">KTZ <\/jats:styled-content><jats:italic>r<\/jats:italic>\u00a0=\u00a00.10, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.54, and <jats:styled-content style=\"fixed-case\">ITZ <\/jats:styled-content><jats:italic>r<\/jats:italic>\u00a0=\u00a00.11, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.48). <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content> values for <jats:styled-content style=\"fixed-case\">ITZ<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content> did not exceed 4\u00a0mg\/L during <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content> therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The majority of feline isolates in Malaysia are thermosensitive. Lack of correlation between E\u2010test and <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content> suggests that the E\u2010test is unreliable to test antifungal susceptibility for <jats:italic>Sporothrix<\/jats:italic> spp. compared to <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content> was the antifungal drug with the lowest <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>. Prolonged <jats:styled-content style=\"fixed-case\">KTZ<\/jats:styled-content> administration may not induce changes in antifungal susceptibility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12417","type":"journal-article","created":{"date-parts":[[2017,1,30]],"date-time":"2017-01-30T02:17:46Z","timestamp":1485742666000},"page":"156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Comparison of two <i>in\u00a0vitro<\/i> antifungal sensitivity tests and monitoring during therapy of <i>Sporothrix schenckii sensu stricto<\/i> in Malaysian cats"],"prefix":"10.1111","volume":"28","author":[{"given":"Hock Siew","family":"Han","sequence":"first","affiliation":[{"name":"Han Veterinary Surgery 50 JLN Molek 2\/2 TMN Molek 81100 Johor Bahru Johor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Kano","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Nihon University College of Bioresource Sciences 1866 Kameino Fujisawa Kanagawa 252\u20100880 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Chen","sequence":"additional","affiliation":[{"name":"Asian Veterinary Specialist Referral Center 109 Section\u00a04 XinYi Road Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Noli","sequence":"additional","affiliation":[{"name":"Servizi Dermatologici Veterinari Strada Bedale della Ressia 2 12016 Peveragno (CN) Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00007-11"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.3.454"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00808-07"},{"key":"e_1_2_5_5_1","first-page":"702","volume-title":"Medical mycology","author":"Kwon\u2010Chung KJ","year":"1992"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2092-2103.2005"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2006.11.004"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12302"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2011.05229.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1112.040891"},{"key":"e_1_2_5_11_1","unstructured":"Clinical and Laboratory Standards Institute.Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: approved standard. 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