{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:45:24Z","timestamp":1743554724331},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,2,5]],"date-time":"2017-02-05T00:00:00Z","timestamp":1486252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007404","name":"Suomen El\u00e4inl\u00e4\u00e4ketieteen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007404","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The house dust mites (HDM) <jats:italic>Dermatophagoides farinae<\/jats:italic> and <jats:italic>D. pteronyssinus<\/jats:italic> are important environmental allergens implicated in the pathogenesis of human and canine atopic dermatitis. Sensitization to HDM measured by allergen\u2010specific IgE is common in Finnish atopic dogs. Studies on HDM prevalence in Finland are few but suggest that HDM are scarce.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of the present study was to evaluate the prevalence of HDM in the home environments of atopic dogs in Finland.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Dust samples were obtained from the homes of 50 atopic dogs. Samples were collected by vacuuming the owners\u2019 mattresses and each dog's bed. In each case, an area of 21 \u00d7 30 cm was vacuumed for 2 min. Samples weighing 100 mg or more were considered sufficient for determination of HDM allergen concentrations (Der f 1 and Der p 1) using standardized ELISA. Samples sufficient for further analysis were also examined by direct microscopy for the presence of mites and by multiplex PCR for HDM DNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighty one samples were sufficient for analysis by ELISA, 59 by PCR and 29 by direct microscopy. A single sample was analysed from four homes in which the dog shared the owner's bed. Der f 1 was detected in three samples (3.7%). Der p 1 was not detected in any sample. No mites were identified on microscopy. Five samples were positive for HDM on multiplex PCR (8.4%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>House dust mites seem to be uncommon in the home environment of atopic dogs in Finland despite reported frequent allergen\u2010specific IgE antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12421","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T04:12:45Z","timestamp":1486354365000},"page":"225","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prevalence of house dust mites in the homes of atopic dogs in Finland"],"prefix":"10.1111","volume":"28","author":[{"given":"Kirsti J. M.","family":"Schildt","sequence":"first","affiliation":[{"name":"Animagi Aurinkolahti Solvikinkatu 13 00990 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riitta T. K.","family":"Sepp\u00e4nen","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital University of Helsinki Koetilantie 2 00014 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna K.","family":"Hielm\u2010Bj\u00f6rkman","sequence":"additional","affiliation":[{"name":"Department of Equine and Small Animal Medicine University of Helsinki Koetilantie 2 00014 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leena E.","family":"Saijonmaa\u2010Koulumies","sequence":"additional","affiliation":[{"name":"Veterinary Teaching Hospital University of Helsinki Koetilantie 2 00014 Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Belova","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Science University of Life Sciences Kreutzwaldi 1 51014 Tartu Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2003.64.1580"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2005.00461.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.1999.00850.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-002-0074-2"},{"key":"e_1_2_6_6_1","unstructured":"PalolahtiJ. Vetlab Heska Immucept\u00ae results of serological allergen tests of dogs in Finland during the years 2008\u20132014 (personal communication)."},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01595.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03870.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00758.x"},{"key":"e_1_2_6_10_1","first-page":"224","article-title":"Multiplex PCR for identifying common dust mites species (Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blomia tropicalis)","volume":"30","author":"Thet\u2010Em T","year":"2012","journal-title":"Asian Pac J All Immunol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb04993.x"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12421","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T17:43:17Z","timestamp":1696614197000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,5]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1111\/vde.12421"],"URL":"https:\/\/doi.org\/10.1111\/vde.12421","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,5]]},"assertion":[{"value":"2016-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}