{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:25:08Z","timestamp":1740126308781,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T00:00:00Z","timestamp":1488412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria IUSA, Spain"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Two studies have observed that growth media containing gentamicin can inhibit the growth of the yeast organism <jats:italic>Malassezia pachydermatis<\/jats:italic>. The minimum inhibitory concentration (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>) of this bactericidal antibiotic for this organism has not been previously determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the susceptibility of <jats:italic>M. pachydermatis<\/jats:italic> isolates to gentamicin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> of gentamicin was determined using a modified version of the M27\u2010A3 microdilution method following the guidelines of the Clinical and Laboratory Standards Institute. A modified Christensen's urea broth was used to enhance the growth of the <jats:italic>M. pachydermatis<\/jats:italic> isolates. Visual and spectrophotometric end\u2010point readings were performed to detect the presence or absence of yeast growth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>50 and <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>90 of gentamicin were 8.12 \u03bcg\/mL and 32.5 \u03bcg\/mL, respectively; <jats:italic>M. pachydermatis<\/jats:italic> strains were classified as susceptible (S), intermediate (I) and resistant (R). The susceptibility of these isolates to gentamicin <jats:italic>in vitro,<\/jats:italic> by visual and spectrophotometric end\u2010point reading, was: S, 54\u201356%; I, 40\u201341%; and R, 3\u20136%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Prospective MICs for <jats:italic>M. pachydermatis<\/jats:italic> have been established for gentamicin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12427","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T08:12:44Z","timestamp":1488442364000},"page":"348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i> susceptibility testing of <i>Malassezia pachydermatis<\/i> to gentamicin"],"prefix":"10.1111","volume":"28","author":[{"given":"Freddy A.","family":"Silva","sequence":"first","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA) Universidad de Las 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