{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:32:12Z","timestamp":1791588732396,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T00:00:00Z","timestamp":1502841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cutaneous squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content>) is an uncommon disease affecting koi carp (<jats:italic>Cyprinus carpio<\/jats:italic>). Cutaneous papilloma (carp pox) is a benign epidermal proliferation reported in koi and has been shown to be caused by Cyprinid Herpesvirus 1 (Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>Histological, ultrastructural and molecular investigations were carried out aiming to investigate the aetiology of <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> within archived tissue samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Surgical samples of masses located on the integument, fins and lips of 13 koi carp belonging to different private owners were included in this retrospective study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>1 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> presence were investigated in five <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> formalin\u2010fixed paraffin\u2010embedded tissue samples to recognize Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>1 presence and its replication activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All cases were histologically diagnosed as <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content>. The ultrastructural observations confirmed the squamous differentiation of neoplastic epithelial cells, which showed abundant tonofilament bundles and desmosomes. Although no virus particles were revealed ultrastructurally, the molecular investigation detected viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> in five epidemiologically unrelated <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content>. Viral transcript analysis revealed no evidence for viral replication in the tested <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content>, which could be consistent with latent infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>These findings illustrate the frequent association of carp <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> with Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>1, although a direct cause\u2013effect relationship cannot be established at this time. Therefore, surveillance programmes should take into account the suspected viral origin of <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> to better inform prevention and control of Cy<jats:styled-content style=\"fixed-case\">HV<\/jats:styled-content>1 in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12482","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T07:20:42Z","timestamp":1502954442000},"page":"60","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Detection of Cyprinid Herpesvirus 1 <scp>DNA<\/scp> in cutaneous squamous cell carcinoma of koi carp (<i>Cyprinus carpio<\/i>)"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8603-8418","authenticated-orcid":false,"given":"Rubina","family":"Sirri","sequence":"first","affiliation":[{"name":"Department of Veterinary Medical Sciences University of Bologna Via Tolara 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