{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:25:12Z","timestamp":1740126312353,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T00:00:00Z","timestamp":1515369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Stallergenes Greer"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2018,4]]},"abstract":"Background<\/jats:title>Percutaneous testing (PT<\/jats:styled-content>) is preferred to intradermal testing in humans for the in vivo<\/jats:italic> identification of allergen hypersensitivity, but the methodology has not been well described for use in dogs with atopic dermatitis.<\/jats:p><\/jats:sec>Hypothesis\/Objectives<\/jats:title>To identify the irritant threshold concentrations (ITC<\/jats:styled-content>) of eight aeroallergens using a commercial prick test device in normal dogs.<\/jats:p><\/jats:sec>Animals<\/jats:title>Twenty healthy, privately owned dogs.<\/jats:p><\/jats:sec>Methods<\/jats:title>Percutaneous testing was performed using the GREER<\/jats:styled-content>\u00ae<\/jats:sup> Pick\u00ae<\/jats:sup> System (Stallergenes Greer; Lenoir, NC<\/jats:styled-content>, USA<\/jats:styled-content>). Five dilutions of glycerinated extracts of Bromis inermis<\/jats:italic>, Sorghum halepense<\/jats:italic>, Chenopodium album<\/jats:italic>, Ambrosia psilostachya<\/jats:italic>, Salix nigra<\/jats:italic> and Acer negundo<\/jats:italic>, as well as four dilutions of Dermatophagoides farinae<\/jats:italic> and D. pteronyssinus<\/jats:italic> were included. Glycerinated histamine (6 mg\/ml<\/jats:styled-content>) and glycerin\/Coca's solution were used for the positive and negative controls, respectively. Orthogonal wheal diameters were measured for each test site every 5 min for 25 min. Reactions were considered significant when the average wheal diameter was equal to or greater than the mean of the positive and negative controls.<\/jats:p><\/jats:sec>Results<\/jats:title>Significant reactions were noted in five of 20 (25%) of dogs. The ITC<\/jats:styled-content> (\u226410% of dogs reacting) were 1:20 w\/v for B. inermis<\/jats:italic> and S. nigra<\/jats:italic>, 1:400 w\/v for D. farinae<\/jats:italic> and 1:200 w\/v for D. pteronyssinus<\/jats:italic>.<\/jats:p><\/jats:sec>Conclusions and clinical importance<\/jats:title>These results suggest that the pollen allergens evaluated in this study can be used for PT<\/jats:styled-content> at their undiluted concentration (1:20 w\/v) with a reasonable assurance of few false positive reactions in dogs. Dust mites require dilution for testing at the ITC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12514","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T10:18:21Z","timestamp":1515579501000},"page":"117","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Percutaneous prick test irritant threshold concentrations for eight allergens in healthy nonsedated dogs in the USA<\/scp>"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9084-0913","authenticated-orcid":false,"given":"Matthew J.H.","family":"Carnett","sequence":"first","affiliation":[{"name":"SkinVet Clinic Suite 120, 15800 SW Upper Boones Ferry Road Lake Oswego OR 97035 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0219-0242","authenticated-orcid":false,"given":"Jon D.","family":"Plant","sequence":"additional","affiliation":[{"name":"SkinVet Clinic Suite 120, 15800 SW Upper Boones Ferry Road Lake Oswego OR 97035 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,8]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"376","volume-title":"Muller and Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00313-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02728.x"},{"key":"e_1_2_6_5_1","first-page":"75","article-title":"Systemic reactions from skin testing: literature review","volume":"16","author":"Liccardi G","year":"2006","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70419-7"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2004.00400.x"},{"volume-title":"Allergic Skin Diseases of Dogs and Cats","year":"1997","author":"Reedy LM","key":"e_1_2_6_8_1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00797.x"},{"issue":"1","key":"e_1_2_6_10_1","first-page":"71","article-title":"Determination of the optimal threshold concentrations of eight allergens for skin percutaneous (prick) test in dogs","volume":"27","author":"Lorente C","year":"2016","journal-title":"Vet Dermatol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12320"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12464"},{"key":"e_1_2_6_13_1","first-page":"428","article-title":"Vergleich von Intrakutan\u2010 und Pricktest in der Allergiediagnostik beim Hund [In German: Comparison of the intradermal and prick tests for diagnosis of allergy in the dog]","volume":"19","author":"Ballauf B","year":"1991","journal-title":"Tier\u00e4rztl Prax"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12054"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12343"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0514-6"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70409-9"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12514","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12514","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T07:21:56Z","timestamp":1695540116000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12514"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,8]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/vde.12514"],"URL":"https:\/\/doi.org\/10.1111\/vde.12514","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"type":"print","value":"0959-4493"},{"type":"electronic","value":"1365-3164"}],"subject":[],"published":{"date-parts":[[2018,1,8]]},"assertion":[{"value":"2017-11-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}