{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:14:33Z","timestamp":1787620473319,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003766","name":"Ministerul Educatiei Nationale","doi-asserted-by":"publisher","award":["NRF\u20102015R1D1A1A01060235"],"award-info":[{"award-number":["NRF\u20102015R1D1A1A01060235"]}],"id":[{"id":"10.13039\/501100003766","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is a challenging pathogen cultured from cases of acute and chronic canine otitis and sometimes in cases of deep pyoderma. The spread of antimicrobial resistance, especially carbapenem resistance, is a serious therapeutic challenge worldwide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To investigate the identification and characterization of resistant <jats:italic>P. aeruginosa<\/jats:italic> clinical canine isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials<\/jats:title><jats:p>Clinical isolates (n = 80) were collected from dogs with pyoderma (n = 18) and otitis (n = 62) in Korea.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Antimicrobial susceptibility was determined using agar dilution and using Clinical and Laboratory Standards Institute guidelines for recording susceptibility for human <jats:italic>Pseudomonas<\/jats:italic> isolates; genetic relatedness of isolates was investigated by multilocus sequence typing (<jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content>) and <jats:italic>Spe<\/jats:italic>I macrorestriction analysis. The class 1 integrons were amplified and sequenced using primer walking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Most isolates were susceptible to colistin (97.5%), polymyxin B (96.3%), ciprofloxacin (81.3%) and meropenem (80.0%); whereas resistance to aztreonam (80%), piperacillin (52.5%), piperacillin\/tazobactam (41.3%) and cefepime (37.5%) was high; 12 carbapenem\u2010nonsusceptible isolates (15%) were detected. <jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content> revealed 45 different sequence types (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>s) and macrorestriction analysis detected 55 distinct pulsotypes (<jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content>s), which were divided into 25 clonal groups. Among carbapenem\u2010nonsusceptible isolates, 10 (83.3%) were <jats:styled-content style=\"fixed-case\">VIM<\/jats:styled-content>\u20102\u2010producing strains. Nine <jats:styled-content style=\"fixed-case\">VIM<\/jats:styled-content>\u20102\u2010producing isolates were identified as <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>1047 and harboured the same 2.8 kb class 1 integron. One remaining isolate was <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>1203 with 2.1 kb class 1 integron.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>This study demonstrated the diversity of the phenotype and genotype of clinical <jats:italic>P. aeruginosa<\/jats:italic> isolates from dogs with pyoderma and otitis. The identification of <jats:styled-content style=\"fixed-case\">VIM<\/jats:styled-content>\u20102\u2010producing <jats:italic>P. aeruginosa<\/jats:italic> in dogs is alarming and warrants further surveillance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12534","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T17:58:29Z","timestamp":1522000709000},"page":"186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Identification of <scp>VIM<\/scp>\u20102 metallo\u2010\u03b2\u2010lactamase\u2010producing <i>Pseudomonas aeruginosa<\/i> isolated from dogs with pyoderma and otitis in Korea"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1157-2237","authenticated-orcid":false,"given":"Jae\u2010Eun","family":"Hyun","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul 08826 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae\u2010Ho","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Companion Animal and Animal Resources Science Joongbu University Chungnam 32713 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7113-0361","authenticated-orcid":false,"given":"Cheol\u2010Yong","family":"Hwang","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University Seoul 08826 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2009.04.020"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2006.00550.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00575.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh332"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3538-3540.2005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.4.891-897.2000"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt269"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12655"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3947\/ic.2015.47.1.33"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252308001485"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.01.008"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.d1540"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt066"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.101070"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.225.548"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12347"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2000.00336.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku133"},{"key":"e_1_2_6_21_1","unstructured":"CLSI.Performance standards for antimicrobial susceptibility testing: twenty fourth informational supplement. 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