{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:39Z","timestamp":1787016159773,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T00:00:00Z","timestamp":1526860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Agria och SKK Forskningsfond"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                      is genotypically diverse within the canine population and multiple strains may colonize individual dogs at any given time. If multiple strains with distinct antimicrobial resistance profiles are present in superficial bacterial folliculitis (\n                      <jats:styled-content style=\"fixed-case\">SBF<\/jats:styled-content>\n                      ), sampling a single skin lesion for culture and antimicrobial susceptibility testing (\n                      <jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content>\n                      ) might be inadequate to select effective therapy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Hypothesis\/Objectives<\/jats:title>\n                    <jats:p>\n                      To investigate\n                      <jats:italic>S. pseudintermedius<\/jats:italic>\n                      diversity in carriage sites and lesions of dogs with\n                      <jats:styled-content style=\"fixed-case\">SBF<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>\n                      Fourteen dogs with\n                      <jats:styled-content style=\"fixed-case\">SBF<\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                      isolates obtained from perineum, gingiva and four to six skin lesions per dog were subjected to pulsed\u2010field gel electrophoresis (\n                      <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>\n                      ) and\n                      <jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content>\n                      to assess diversity between lesions. For two dogs, 14\u201316 isolates per lesion were included in the analysis to assess diversity within lesions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Analysis of one isolate per lesion revealed one to four strains displaying unique\n                      <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>\n                      profiles, and up to three unique antimicrobial resistance (\n                      <jats:styled-content style=\"fixed-case\">AMR<\/jats:styled-content>\n                      ) profiles for each dog. Multiple pustules from the same dog always harboured the same strain, whereas papules, crusts and collarettes did not. Up to four strains with distinct\n                      <jats:styled-content style=\"fixed-case\">AMR<\/jats:styled-content>\n                      profiles were isolated from the same lesion in two dogs. In 12 dogs, at least one carriage site strain also was represented in lesions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical importance<\/jats:title>\n                    <jats:p>\n                      Lesions of\n                      <jats:styled-content style=\"fixed-case\">SBF<\/jats:styled-content>\n                      may harbour multiple\n                      <jats:italic>S. pseudintermedius<\/jats:italic>\n                      strains with distinct antimicrobial resistance profiles. Pustules are the best target for bacterial culture. It remains unclear whether isolation of different strains from other lesion types is a consequence of contamination or co\u2010infection by multiple strains.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vde.12549","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T05:15:24Z","timestamp":1526879724000},"page":"291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Diversity of\n                    <i>Staphylococcus pseudintermedius<\/i>\n                    in carriage sites and skin lesions of dogs with superficial bacterial folliculitis: potential implications for diagnostic testing and therapy"],"prefix":"10.1111","volume":"29","author":[{"given":"Rikke Friis","family":"Larsen","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Copenhagen Stigb\u00f8jlen 4 1870 Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Boysen","sequence":"additional","affiliation":[{"name":"Veterinary Dermatology Clinic Kongevejen 159 2830 Virum Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisbeth Rem","family":"Jessen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Copenhagen Dyrl\u00e6gevej 16 1870 Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Guardabassi","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Copenhagen Stigb\u00f8jlen 4 1870 Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-5996","authenticated-orcid":false,"given":"Peter","family":"Damborg","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Copenhagen Stigb\u00f8jlen 4 1870 Frederiksberg C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq543"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.155.9.259"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.5326\/0380407"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.11.014"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01046.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.8.1337"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2010.00894.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00828.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/BF03547307"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.5.535"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.101069"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12118"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12414"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.06.012"},{"key":"e_1_2_7_16_1","unstructured":"CLSI.Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals\u20103rd ed. 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