{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:25:04Z","timestamp":1785774304451,"version":"3.56.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,8,8]],"date-time":"2018-08-08T00:00:00Z","timestamp":1533686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Otitis externa is a common multifactorial disease with a prevalence in dogs as high as 10\u201320%. In humans, the diversity of the cutaneous mycobiota appears to increase in diseased states, whereas one canine study identified a decrease in diversity of the cutaneous mycobiota in atopic dogs compared to healthy individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/objectives<\/jats:title><jats:p>To describe the otic mycobiota in dogs with otitis externa compared to healthy dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Samples were collected from six dogs with clinical and cytological evidence of otitis externa and five clinically normal dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Swabs were collected from the ears of six dogs with fungal otitis externa. DNA from each sample was isolated and Illumina sequencing was performed targeting the internal transcribed spacer region. Sequences were processed using the bioinformatics software MOTHUR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fungi from ten different phyla were identified. The mycobiota of all affected ears was dominated by the genera<jats:italic>Malassezia,<\/jats:italic>which accounted for 55.7\u201398.4% of sequences (median 96.8%). Affected ears had significantly decreased observed richness, estimated richness and inverse Simpson's diversity index compared to controls (<jats:italic>P<\/jats:italic>= 0.008). Linear discriminant analysis effect size (LEfSe) analysis identified 42 operational taxonomic units (OTUs) that were differentially abundant (<jats:italic>P<\/jats:italic>&lt; 0.05). Three OTUs were over\u2010represented in the affected ears, including<jats:italic>M. pachydermatis,<\/jats:italic>whereas 39 OTUs were over\u2010represented in healthy ears.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Reduced fungal richness and diversity was present in affected ears, with markedly higher relative abundances of<jats:italic>Malassezia<\/jats:italic>. The otic fungal mycobiota is much more complex than has been identified with culture\u2010based studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12665","type":"journal-article","created":{"date-parts":[[2018,8,8]],"date-time":"2018-08-08T07:49:49Z","timestamp":1533714589000},"page":"417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Analysis of the otic mycobiota in dogs with otitis externa compared to healthy individuals"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8933-0115","authenticated-orcid":false,"given":"Juraj","family":"Korbelik","sequence":"first","affiliation":[{"name":"Department of Pathobiology Ontario Veterinary College University of Guelph 50 Stone Road E Guelph ON Canada N1G 2W1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameet","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies Ontario Veterinary College University of Guelph 50 Stone Road E Guelph ON Canada N1G 2W1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Ontario Veterinary College University of Guelph 50 Stone Road E Guelph ON Canada N1G 2W1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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