{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:22:55Z","timestamp":1789122175078,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,8,23]],"date-time":"2018-08-23T00:00:00Z","timestamp":1534982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nestle Purina PetCare"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"Background<\/jats:title>Juvenile onset generalized demodicosis (JOGD<\/jats:styled-content>) is thought to occur due to immunological abnormalities and is over\u2010represented in pit bull terrier\u2010type dogs.<\/jats:p><\/jats:sec>Animals<\/jats:title>Twelve pit bull terrier\u2010type dogs with JOGD<\/jats:styled-content> and 12 age\u2010matched healthy pit bull terrier\u2010type dogs.<\/jats:p><\/jats:sec>Objective<\/jats:title>To investigate immunological differences between age\u2010matched healthy and JOGD<\/jats:styled-content> pit bull terrier\u2010type dogs by flow cytometry, multiplex, molecular and serological assays.<\/jats:p><\/jats:sec>Methods and materials<\/jats:title>Flow cytometry quantified B cells expressing MHCII<\/jats:styled-content> or surface\u2010bound IgG, CD<\/jats:styled-content>4+ T cells expressing MHCII<\/jats:styled-content>, CD<\/jats:styled-content>8 T cells expressing MHCII<\/jats:styled-content> or CD<\/jats:styled-content>11a, neutrophil and monocyte markers. Surface expression was quantified by calculating the geometric mean fluorescence index. The Wilcoxon rank sum test was used to compare median results for IL<\/jats:styled-content>\u20102, IL<\/jats:styled-content>\u20104, IL<\/jats:styled-content>\u20106, IL<\/jats:styled-content>\u20107, IL<\/jats:styled-content>\u20108, IL<\/jats:styled-content>\u201010, IL<\/jats:styled-content>\u201013, IL<\/jats:styled-content>\u201018, FOXP<\/jats:styled-content>3, monocyte chemoattractant protein\u20101, GM<\/jats:styled-content>\u2010CSF<\/jats:styled-content>, KC<\/jats:styled-content>, IgE, IgA, IgG, IgM, C\u2010reactive protein, lymphocyte, neutrophil and monocyte in the groups. IFN<\/jats:styled-content>\u2010gamma, IP<\/jats:styled-content>\u201010, IL<\/jats:styled-content>\u201015, IL<\/jats:styled-content>\u201031 and TNF<\/jats:styled-content>\u2010alpha also were measured; however, insufficient dogs (<5) had values that were in range of the assay to allow for statistical evaluation. Significance was defined as P<\/jats:italic>\u00a0<\u00a00.05.<\/jats:p><\/jats:sec>Results<\/jats:title>Serum concentrations of IL<\/jats:styled-content>\u20102, IL<\/jats:styled-content>\u201018 and MCP<\/jats:styled-content>\u20101 were significantly higher (P<\/jats:italic>\u00a0=\u00a00.01, P<\/jats:italic>\u00a0=\u00a00.01, P<\/jats:italic>\u00a0=\u00a00.04) in the JOGD<\/jats:styled-content> group. Also, IgA median value was significantly higher (P<\/jats:italic>\u00a0=\u00a00.002) in pit bull terrier\u2010type dogs with JOGD<\/jats:styled-content>. Flow cytometry revealed that T\u2010cell, neutrophil and monocyte markers were not different between groups.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Results suggest an appropriate compensatory immune response by pit bull terrier\u2010type dogs in the JOGD<\/jats:styled-content> group and do not support the explanation of global immune deficiency in these dogs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12681","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T02:49:00Z","timestamp":1535078940000},"page":"482","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of immunological parameters in pit bull terrier\u2010type dogs with juvenile onset generalized demodicosis and age\u2010matched healthy pit bull terrier\u2010type dogs"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3816-767X","authenticated-orcid":false,"given":"Clarissa P.","family":"Souza","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Schissler","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena T.","family":"Contreras","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven W.","family":"Dow","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leone S.","family":"Hopkins","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan W.","family":"Coy","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rod A.W.","family":"Rosychuk","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Hawley","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Lappin","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University 300 West Drake Road Fort Collins CO 80525 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2010.00922.x"},{"key":"e_1_2_5_3_1","first-page":"307","volume-title":"Muller and Kirk's Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.1996.tb00237.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2010.01463.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(77)90029-2"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(83)90032-2"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(92)90067-Z"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12136"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2010.03255.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182017000774"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000082565"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2000.tb11741.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2005.09.003"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00569.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.08.021"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12125"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.03.014"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2006.12.002"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2004.08.020"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(03)00049-7"},{"key":"e_1_2_5_22_1","first-page":"189","article-title":"Why we (usually) don't have to worry about multiple comparisons","volume":"52","author":"Gelman A","year":"2012","journal-title":"J Res Educ Eff"},{"key":"e_1_2_5_23_1","unstructured":"GelmanA LokenE.The garden of forking paths: Why multiple comparisons can be a problem even when there is no \u201cfishing expedition\u201d or \u201cp\u2010hacking\u201d and the research hypothesis was posited ahead of time. New York: Columbia University Department of Statistics (2013). Available athttp:\/\/www.stat.columbia.edu\/~gelman\/research\/unpublished\/p_hacking.pdfAccessed 21\/06\/2018."},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.7.3706-3715.2004"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.1996.tb00249.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.64.513"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.11.012"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985817725388"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2008.0027"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2010.00228.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2014.05.010"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(94)05298-7"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01218.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2004.01581.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1159\/000323009"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.244.9.1075"},{"key":"e_1_2_5_37_1","first-page":"45","volume-title":"Introduction to error analysis, the study of uncertainties in physical measurements","author":"Taylor JR","year":"1997"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098589703400403"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00842.x"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvde.12681","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12681","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T23:43:25Z","timestamp":1694735005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12681"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,23]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1111\/vde.12681"],"URL":"https:\/\/doi.org\/10.1111\/vde.12681","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,23]]},"assertion":[{"value":"2018-06-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}