{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:58:19Z","timestamp":1784084299428,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,20]],"date-time":"2019-01-20T00:00:00Z","timestamp":1547942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Otitis externa is a common presenting complaint in practice. Ear infections by <jats:italic>Pseudomonas aeruginosa<\/jats:italic> are particularly problematic due to the organism's high level of resistance and ability to damage the tympanum. Treatment should be based on susceptibility testing although minimum inhibitory concentrations (<jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s) are not available for all treatment options. Silver sulfadiazine has been used in cases of recurrent <jats:italic>P.\u00a0aeruginosa<\/jats:italic> otitis, although a <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> for silver sulfadiazine as a single agent has not been established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To describe susceptibility patterns of <jats:italic>P.\u00a0aeruginosa<\/jats:italic> isolated from canine otitis externa and determine the <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> for silver sulfadiazine and other topical antimicrobials.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Thirty\u2010six <jats:italic>P.\u00a0aeruginosa<\/jats:italic> isolates were collected from client\u2010owned dogs, suffering from otitis externa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Susceptibility patterns were established using disc diffusion susceptibility testing against 17 antimicrobial agents. For determination of the <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>, selected strains were tested against increasing concentrations of marbofloxacin, enrofloxacin, gentamicin, polymyxin B and silver sulfadiazine using broth microdilution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For nine of 17 antimicrobial agents, complete resistance was seen in all isolates tested via disk diffusion susceptibility testing. Approximately 94% and 96% of isolates were susceptible to gentamicin and imipenem, respectively. These findings were consistent with broth dilution, where all strains were susceptible to gentamicin. Resistance was higher against polymyxin B and the fluoroquinolones. Silver sulfadiazine was effective <jats:italic>in\u00a0vitro<\/jats:italic> with a <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> ranging from 1 to 64\u00a0\u03bcg\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical significance<\/jats:title><jats:p>As the <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> of silver sulfadiazine was lower than the concentration in a 1% preparation, such a product potentially represents a treatment option for dogs with <jats:italic>P.\u00a0aeruginosa<\/jats:italic> otitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12718","type":"journal-article","created":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T05:29:59Z","timestamp":1548048599000},"page":"145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Determination of minimum inhibitory concentrations for silver sulfadiazine and other topical antimicrobial agents against strains of <i>Pseudomonas aeruginosa<\/i> isolated from canine otitis externa"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9160-3901","authenticated-orcid":false,"given":"Maritta S. 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