{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:01:55Z","timestamp":1783728115173,"version":"3.55.0"},"reference-count":7,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T00:00:00Z","timestamp":1574035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Canine pyoderma is a common skin infection caused predominantly by staphylococcal bacteria. Because of increasing rates of antimicrobial resistance in bacterial isolates, there is an urgent need for alternative or supplementary treatment options. W16P576, a Water Extract of Complex Mix of Edible Plants (WECMEP), has shown <jats:italic>in vitro<\/jats:italic> activity against a variety of bacteria, including <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>. A canine model of pyoderma was developed which allows <jats:italic>in vivo<\/jats:italic> testing of antimicrobial agents in a controlled environment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the antibacterial efficacy of topical application of W16P576 in a model of canine pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Nine laboratory housed beagle dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>In an evaluator\u2010blinded cross\u2010over study with an eight week washout period, dogs were treated topically twice daily with W16P576 WECMEP or its vehicle, starting three days before bacterial challenge. On the day of challenge, each dog was treated with two concentrations of a clinical <jats:italic>S<\/jats:italic>.\u00a0<jats:italic>pseudintermedius<\/jats:italic> strain on opposite sides of the body. Topical treatment was continued for 11\u00a0days and lesions of pyoderma were evaluated and scored for 14\u00a0days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All dogs developed lesions consistent with bacterial pyoderma. Lesion scores were generally higher on the side inoculated with a higher concentration of bacteria. Treatment with W16P576 significantly reduced lesion development and hastened resolution of lesions, compared to placebo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Topical application of W16P576 markedly reduced lesion development in this proof of principle study. Clinical trials are warranted to estimate benefits for dogs with naturally occurring pyoderma under field conditions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12808","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T10:09:20Z","timestamp":1574071760000},"page":"86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Efficacy study of a topical treatment with a plant extract with antibiofilm activities using an in vivo model of canine superficial pyoderma"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4346-5911","authenticated-orcid":false,"given":"Wolfgang","family":"B\u00e4umer","sequence":"first","affiliation":[{"name":"Department of Molecular Biomedical Sciences College of Veterinary Medicine North Carolina State University 1060 William Moore Drive Raleigh NC 27607 USA"},{"name":"Institute of Pharmacology and Toxicology Faculty of Veterinary Medicine Freie Universit\u00e4t Berlin Koserstra\u00dfe 20 14195 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathology College of Veterinary Medicine North Carolina State University 1060 William Moore Drive Raleigh NC 27607 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6423-7718","authenticated-orcid":false,"given":"Chie","family":"Tamamoto\u2010Mochizuki","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine North Carolina State University 1060 William Moore Drive Raleigh NC 27607 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.102651"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01046.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13362"},{"key":"e_1_2_6_5_1","first-page":"365","article-title":"A sophisticated plant extract inhibits in vitro growth and biofilm formation of Staphylococcus \n                  pseudintermedius isolated from canine skin and ear infections","volume":"29","author":"Leotoing L","year":"2018","journal-title":"Vet Dermatol"},{"key":"e_1_2_6_6_1","unstructured":"CLSI.Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals\u2013 3rd ed. CLSI document VET01S. Wayne PA: Clinical and Laboratory Standards Institute;2015."},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2736\/jjvd.14.71"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12374"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vde.12808","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.12808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T19:52:25Z","timestamp":1693857145000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.12808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,18]]},"references-count":7,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1111\/vde.12808"],"URL":"https:\/\/doi.org\/10.1111\/vde.12808","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,18]]},"assertion":[{"value":"2019-09-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}