{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T02:36:43Z","timestamp":1785811003249,"version":"3.56.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Staphylococcus pseudintermedius<\/jats:italic> is a major bacterial species associated with canine pyoderma and otitis. Fusidic acid is used to treat skin infections caused by Gram\u2010positive bacteria. The incidence of resistance to fusidic acid in <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> has importance in terms of limiting treatment options for bacterial infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/objectives<\/jats:title><jats:p>To evaluate the occurrence and mechanisms of fusidic acid resistance in clinical isolates of <jats:italic>S.\u00a0pseudintermedius<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Fifty\u2010two <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> isolates were collected from dogs with pyoderma (n\u00a0=\u00a036) or otitis (n\u00a0=\u00a016).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>The disk diffusion method determined that isolates &lt;24\u00a0mm were resistant to fusidic acid. Minimum inhibitory concentrations (MICs) were measured by the E\u2010test in those with confirmed resistance to fusidic acid by the disk diffusion method. Phenotypically fusidic acid resistant isolates were subjected to PCR to detect the presence of resistance\u2010related genes (<jats:italic>fusA<\/jats:italic>, <jats:italic>fusB<\/jats:italic>, <jats:italic>fusC<\/jats:italic> and <jats:italic>fusD<\/jats:italic>) and <jats:italic>fusA<\/jats:italic> was further sequenced to identify point mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fourteen of 52 (27%) <jats:italic>S.\u00a0pseudintermediu<\/jats:italic>s isolates were resistant to fusidic acid and all of these showed low\u2010level resistance. Among fusidic acid resistant isolates, <jats:italic>fusA<\/jats:italic> point mutations were confirmed in 11 isolates and amino acid substitutions were found in five. <jats:italic>fusC<\/jats:italic> was detected in seven isolates, but neither <jats:italic>fusB<\/jats:italic> nor <jats:italic>fusD<\/jats:italic> was detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>This study demonstrates the occurrence and resistance mechanisms to fusidic acid in clinical isolates of <jats:italic>S.\u00a0pseudintermedius.<\/jats:italic> Continuous monitoring for fusidic acid resistance is recommended.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12844","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T01:59:01Z","timestamp":1583114341000},"page":"267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification of fusidic acid resistance in clinical isolates of <i>Staphylococcus pseudintermedius<\/i> from dogs in Korea"],"prefix":"10.1111","volume":"31","author":[{"given":"Yun\u2010Ji","family":"Lim","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University 1 Gwanak\u2010ro, Gwanak\u2010gu Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Eun","family":"Hyun","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University 1 Gwanak\u2010ro, Gwanak\u2010gu Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7113-0361","authenticated-orcid":false,"given":"Cheol\u2010Yong","family":"Hwang","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science College of Veterinary Medicine Seoul National University 1 Gwanak\u2010ro, Gwanak\u2010gu Seoul 08826 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01046.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00635.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12382"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)93759-7"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/499365"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.03.010"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(69)90934-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00523-10"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01057.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir170"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(98)00072-7"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02389.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00871-08"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04971.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01542-06"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir164"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"2,048","DOI":"10.1093\/jac\/dkv056","article-title":"Susceptibility in vitro of canine methicillin\u2010resistant and\u2010susceptible staphylococcal isolates to fusidic acid, chlorhexidine and miconazole: opportunities for topical therapy of canine superficial pyoderma","volume":"70","author":"Clark S","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn398"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-019-1449-z"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01232-09"},{"key":"e_1_2_5_22_1","unstructured":"EUCAST (European Committee on Antimicrobial Susceptibility Testing).Breakpoint tables for interpretation of MICs and zone diameters. 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