{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:35:56Z","timestamp":1777649756509,"version":"3.51.4"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In the Netherlands there is a lack of data regarding resistance of <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> to the systemic antimicrobial drugs used for the treatment of superficial pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess antimicrobial resistance, with emphasis on resistance to clindamycin and meticillin, in clinical isolates of <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> isolated from dogs with superficial pyoderma. Results were compared between dogs with and without a history of systemic antimicrobial therapy during the previous year.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>A retrospective study of 237 referral cases presented to an academic teaching hospital between 2014 and 2019, with the clinical and microbiological diagnosis of superficial pyoderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>All clinical isolates were identified primarily by MALDI\u2010TOF mass spectrometry. Antimicrobial susceptibility was tested either by an agar diffusion method (2014\u20132016) or by broth microdilution. Antimicrobial history in the preceding year was obtained from medical records.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Meticillin\u2010resistant <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> (MRSP) was isolated from 8% of superficial pyoderma cases. Within the meticillin\u2010susceptible <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> (MSSP) population, clindamycin resistance was significantly more common in isolates derived from dogs with histories of antimicrobial treatment (37.7%) compared to dogs with no histories of exposure (21.7%; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.03).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Given the high prevalence of clindamycin resistance in MSSP isolated from dogs with prior antimicrobial exposure, it is recommended that bacterial culture and susceptibility testing be pursued before prescribing systemic antimicrobials. Clindamycin should be regarded as the preferred treatment option if susceptibility is confirmed, due to its narrow spectrum and reduced selective pressure for MRSP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12854","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T01:44:45Z","timestamp":1587606285000},"page":"305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Clindamycin resistance of skin derived <i>Staphylococcus pseudintermedius<\/i> is higher in dogs with a history of antimicrobial therapy"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9786-1859","authenticated-orcid":false,"given":"Catharina M.M.","family":"van Damme","sequence":"first","affiliation":[{"name":"Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Yalelaan 1 3584 CM Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1312-6115","authenticated-orcid":false,"given":"Els M.","family":"Broens","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology Faculty of Veterinary Medicine Utrecht University Yalelaan 1 3584 CM Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-646X","authenticated-orcid":false,"given":"Silvia T.","family":"Auxilia","sequence":"additional","affiliation":[{"name":"Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Yalelaan 1 3584 CM Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5846-5340","authenticated-orcid":false,"given":"Yvette M.","family":"Schlotter","sequence":"additional","affiliation":[{"name":"Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Yalelaan 1 3584 CM Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01046.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12444"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12118"},{"key":"e_1_2_6_5_1","unstructured":"Dutch Working Party on Veterinary Antibiotic Policy.WVAB\u2010richtlijn classificatie van veterinaire antimicrobi\u00eble middelen 2015 and Formulary companion animals: dog cat and rabbit 2017. Available at:https:\/\/www.knmvd.nl\/app\/uploads\/sites\/4\/2018\/09\/Formularium\u2010Hond\u2010en\u2010Kat.pdf. Accessed Mar 1 2020."},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.008"},{"key":"e_1_2_6_7_1","unstructured":"CLSI.Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standards 4th edition. CLSI document VET01\u2010A4.Wayne PA:Clinical and Laboratory Standards Institute;2013."},{"key":"e_1_2_6_8_1","unstructured":"CLSI.Performance standards for antimicrobial susceptibility testing 27th edition. CLSI document Supplement M100S.Wayne PA USA:Clinical and Laboratory Standards Institute;2017."},{"key":"e_1_2_6_9_1","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (EUCAST) Breakpoint Tables for Interpretation of MICs and Zone Diameters Version 8.0(2018). 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