{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:41:13Z","timestamp":1771476073604,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009231","name":"European Society of Veterinary Dermatology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009231","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Canine leishmaniosis (CanL) is associated with an aberrant cutaneous immune response. Few studies have compared cutaneous immune cells among dogs with leishmaniosis or infected without leishmaniosis, and noninfected dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objective<\/jats:title><jats:p>Evaluate the number of neutrophils, histiocytes, T lymphocytes, T\u2010bet\u2010positive, GATA3\u2010positive, FoxP3\u2010positive and interleukin (IL)\u201017\u2010positive cells, in lesional (Group A) and normal\u2010looking (Group B) skin of nine dogs with stage II\/III leishmaniosis; in normal\u2010looking PCR\u2010positive (Group C; n\u00a0=\u00a06) or PCR\u2010negative (Group D; n\u00a0=\u00a06) skin of infected dogs; and in the normal\u2010looking skin of 12 noninfected dogs (Group E).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Diagnosis of CanL considered clinical signs, clinicopathological abnormalities, detection of <jats:italic>Leishmania<\/jats:italic> amastigotes in lymph nodes, and\/or bone marrow and positive serological results. Paraffin\u2010embedded skin biopsies were processed for routine immunofluorescence and positive cells were identified using commercially available anti\u2010canine antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In Group A, there was a significantly higher number of neutrophils (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001), histiocytes (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.012), T lymphocytes (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.011), GATA3\u2010positive (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.02) and IL\u201017A\u2010positive (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.002) cells compared to Group E. In Group B, there was a significantly higher number of histiocytes (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.02), T lymphocytes (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.004), GATA3\u2010positive (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.006) and FoxP3\u2010positive (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.028) cells compared to Group E. There was no difference in between groups A and B and between groups C or D and E.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>In the lesional and\/or normal\u2010looking skin of dogs with moderate\/severe CanL there is an infiltration of neutrophils, histiocytes, T lymphocytes, GATA3\u2010, FoxP3\u2010 and IL17A\u2010positive cells. By contrast, the number of these cells is not increased in the normal\u2010looking skin of infected dogs without CanL compared to the skin of noninfected dogs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12922","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T17:10:33Z","timestamp":1609175433000},"page":"99","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the cutaneous inflammatory cells in dogs with leishmaniosis and in dogs without the disease that were naturally infected by <i>Leishmania infantum<\/i> (syn. <i>L. chagasi<\/i>)"],"prefix":"10.1111","volume":"32","author":[{"given":"Andrea","family":"Hernandez\u2010Bures","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine University of Florida 2015 SW 16th Avenue Gainesville FL 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Gillen","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine University of Florida 2015 SW 16th Avenue Gainesville FL 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosmas","family":"Apostolidis","sequence":"additional","affiliation":[{"name":"Clinic of Medicine Faculty of Veterinary Science University of Thessaly Trikalon Str. 224 Karditsa GR\u201043132 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8308-3371","authenticated-orcid":false,"given":"Manolis","family":"Saridomichelakis","sequence":"additional","affiliation":[{"name":"Clinic of Medicine Faculty of Veterinary Science University of Thessaly Trikalon Str. 224 Karditsa GR\u201043132 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Di Loria","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions University \"Federico II\" Via Delpino 1 Napoli 80130 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4893-4289","authenticated-orcid":false,"given":"Domenico","family":"Santoro","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine University of Florida 2015 SW 16th Avenue Gainesville FL 32610 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-86"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00823.x"},{"key":"e_1_2_6_4_1","first-page":"26","volume-title":"Canine leishmaniasis: an update. 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