{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T07:19:03Z","timestamp":1768979943352,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Malassezia pachydermatis<\/jats:italic> is a commensal organism of the skin, yet it may induce dermatitis and\/or otitis. Lactoferricin (Lfcin) is an antimicrobial peptide obtained by the pepsin\u2010mediated digestion of lactoferrin, a multifunctional innate\u2010defence milk protein. The antibacterial activity of Lfcin is thought to cause alteration of bacterial membrane permeability, thus inducing cell death.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to evaluate <jats:italic>in vitro<\/jats:italic> antifungal activity of different dilutions of Lfcin solution against <jats:italic>M<\/jats:italic>.\u00a0<jats:italic>pachydermatis<\/jats:italic> strains isolated from ears of dogs and cats with otitis externa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Fifty clinical <jats:italic>Malassezia<\/jats:italic> isolates from 40 dogs and 10 cats were tested. A dilution method in microtitre plates was used starting with a 20% Lfcin water solution. The different dilutions were 2:1 (13.3%), 1:1 (10%), 1:2 (6.7%), 1:5 (3.3%) and 1:10 (1.8%). Results were expressed as the value at which the growth of 50% (minimal fungicidal concentration MFC<jats:sub>50<\/jats:sub>) and 90% (MFC<jats:sub>90<\/jats:sub>) of yeast cells was inhibited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All strains showed susceptibility to 20% Lfcin solution (100%). With 1:5 and 1:10 dilutions corresponding to 3.3% and 1.8% Lfcin solutions, all strains showed resistance. The MFC<jats:sub>50<\/jats:sub> and MFC<jats:sub>90<\/jats:sub> values were observed at 13.3% and 20% Lfcin solutions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The results indicated that Lfcin solution exhibits the antimicrobial activity specific to antimicrobial peptides. In particular, the 20% solution can be effective in killing <jats:italic>M.\u00a0pachydermatis<\/jats:italic> isolated strains. Some susceptibilities also are evident at lower concentrations of \u22646.7% for four strains.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12973","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T16:23:04Z","timestamp":1621441384000},"page":"316","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vitro<\/i> activity of lactoferricin solution against <i>Malassezia pachydermatis<\/i> from otitis externa in dogs and cats"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2304-751X","authenticated-orcid":false,"given":"Antonio","family":"Corona","sequence":"first","affiliation":[{"name":"Citt\u00e0 di Torino\u201d Veterinary Clinic c.so Traiano 99\/c 10135 Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Vercelli","sequence":"additional","affiliation":[{"name":"Citt\u00e0 di Torino\u201d Veterinary Clinic c.so Traiano 99\/c 10135 Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natascia","family":"Bruni","sequence":"additional","affiliation":[{"name":"Pharmaceutical Institute \u201cCandioli\u201d Via Manzoni 2 10092 Beinasco Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleonora","family":"Guidi","sequence":"additional","affiliation":[{"name":"Citt\u00e0 di Torino\u201d Veterinary Clinic c.so Traiano 99\/c 10135 Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"Cornegliani","sequence":"additional","affiliation":[{"name":"Citt\u00e0 di Torino\u201d Veterinary Clinic c.so Traiano 99\/c 10135 Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.04.020"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-12173-9_3"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00021-11"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2005.05.006"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780600962718"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4868-4869.2004"},{"key":"e_1_2_5_8_1","first-page":"295","article-title":"Malassezia pachydermatis: a review","volume":"37","author":"Guillot J","journal-title":"Med Mycol"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12834"},{"key":"e_1_2_5_10_1","first-page":"S2","article-title":"Principales enfermedades f\u00fangicas que afectan a los animales dom\u00e9sticos","volume":"17","author":"Garc\u00eda ME","year":"1999","journal-title":"Rev Iberoam Micol"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2009.12.012"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X13512119"},{"key":"e_1_2_5_13_1","unstructured":"DaigleJC.Clinical clues diagnosis and treatment ofMalassezia dermatitis. 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