{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:28:13Z","timestamp":1788298093929,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Serological allergen testing (SAT) is used widely to formulate allergen\u2010specific immunotherapy for atopic dogs. Serum immunoglobulin (Ig)E specific for cross\u2010reactive carbohydrate determinants (CCD) can produce false\u2010positive reactions, creating discrepancy between SAT and intradermal allergen test (IDAT) results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The primary objective was to determine if inhibition of anti\u2010CCD IgE in a commercial assay improved correlation with IDAT. The secondary objective was to assess the influence of dog\u2010 and clinic\u2010specific factors, environmental factors, putative allergen exposure and prior medications on intradermal and SAT reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Two\u2010hundred and eleven client\u2010owned dogs were enrolled from eight North American dermatology specialty practices.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Collection of serum samples and IDAT were performed on the same day. Sera were assayed for detection of IgE specific to 25 allergens, before and after treatment with a proprietary inhibitor of anti\u2010CCD IgE. Data for each dog were collected via a questionnaire filled out by veterinary personnel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The correlation between the testing modalities was fair before (Spearman\u2019s rho, \u03c1\u00a0=\u00a00.2092) and after (\u03c1\u00a0=\u00a00.3042) inhibition of anti\u2010CCD IgE. Ciclosporin dose (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.003), independent of duration of use, and duration of lokivetmab use (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001), independent of dose administered, were associated with statistically significant decreases in IgE concentrations across all allergen types.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Contrary to previous reports, this study demonstrated unchanged correlation between SAT and IDAT after inhibition of anti\u2010CCD IgE. Ciclosporin dose and lokivetmab treatment duration may have unexplored effects on IgE concentration during SAT.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.12974","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T13:55:38Z","timestamp":1623246938000},"page":"347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Multivariable analysis of the influence of cross\u2010reactive carbohydrate determinant inhibition and other factors on intradermal and serological allergen test results: a prospective, multicentre study"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5595-5566","authenticated-orcid":false,"given":"Peter","family":"Canning","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences and Advanced Medicine School of Veterinary Medicine University of Pennsylvania 3900 Spruce Street Philadelphia PA 19104 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