{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:45:17Z","timestamp":1790624717788,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T00:00:00Z","timestamp":1628726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Conjunctivitis in atopic dogs has already been described yet is rarely observed, and likely underdiagnosed in practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assay various cytokines in tears and conjunctivae from atopic and normal dogs, and to compare canine atopic dermatitis\u2010associated conjunctivitis with controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Ten atopic and ten normal client\u2010owned dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Ocular surfaces were sampled bilaterally in a prospective study, using two different methods. Tear samples were obtained with a sterile swab previously moistened with saline solution (method A). Conjunctival impressions were obtained with a conjunctival impression device (method B). For each sample, the concentrations of a panel of 13 cytokines were measured by multiplex analyses. CADESI\u20104, pruritus (PS) and conjunctival (CS) scores were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the measured cytokines, only granulocyte\u2010macrophage colony\u2010stimulating factor (GM\u2010CSF), keratinocyte\u2010derived chemokine (KC)\u2010like and interleukin (IL)\u20108 were above the limit of quantification in most samples. Absolute amounts of each cytokine were always higher in samples obtained with method A than with method B. GM\u2010CSF amounts were lower in atopic dogs (method A, P=0.02; method B, P=0.0005). KC levels were higher in atopic dogs, yet the differences were not significant. IL\u20108 amounts were higher in atopic dogs (method A, P=0.0003; method B, P=0.006).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Regardless of the method, these preliminary results suggest an overexpression of IL\u20108 in conjunctivae and tears of atopic dogs despite subtle conjunctival symptoms. As IL\u20108 is commonly found in many inflammatory conditions, further studies are needed to determine its specificity in atopic conjunctivitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13010","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T05:45:39Z","timestamp":1628833539000},"page":"625","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Survey of cytokines on ocular surfaces of atopic dogs by multiplex analysis using two sampling methods \u2013 a pilot study"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3908-7460","authenticated-orcid":false,"given":"Charline","family":"Pressanti","sequence":"first","affiliation":[{"name":"Small Animal Clinic Universit\u00e9 de Toulouse ENVT 23, chemin des Capelles Toulouse 31076 France"},{"name":"UDEAR (INSERM) Universit\u00e9 de Toulouse CHU Purpan \u2013 BP 3028 Toulouse 31024 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Ravailhe","sequence":"additional","affiliation":[{"name":"Small Animal Clinic Universit\u00e9 de Toulouse ENVT 23, chemin des Capelles Toulouse 31076 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5631-4653","authenticated-orcid":false,"given":"Jessie","family":"Castellote\u2010Brun","sequence":"additional","affiliation":[{"name":"Small Animal Clinic Universit\u00e9 de Toulouse ENVT 23, chemin des Capelles Toulouse 31076 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9546-7310","authenticated-orcid":false,"given":"Nicolas","family":"Amalric","sequence":"additional","affiliation":[{"name":"Synelvia 516 Rue Pierre et Marie Curie Lab\u00e8ge 31670 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