{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:18:10Z","timestamp":1775909890289,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In human medicine, narrow\u2010band ultraviolet B (NB\u2010UVB) phototherapy has been used to treat various T\u2010cell\u2010mediated skin diseases. However, the effect of NB\u2010UVB on inflamed canine skin remains uncertain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the effect of NB\u2010UVB phototherapy on the skin of dogs with hapten\u2010induced contact dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Seven healthy beagles without skin problems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Dogs were irradiated with varying doses of NB\u2010UVB to determine the minimal erythema dose (MED). After determining the MEDs of six dogs (excluding one of the seven whose skin did not show a visible reaction), we investigated the effect of NB\u2010UVB on their inflamed skin by topically applying 2,4\u2010dinitrochlorobenzene (DNCB), which causes type 1 helper T cell (Th1)\u2010 and cytotoxic T\u2010cell (Tc)1\u2010induced skin inflammation. We then irradiated the skin with NB\u2010UVB. We analysed the treated skin samples via histopathological and immunohistochemical methods, and TdT\u2010mediated dUTP nick\u2010end labelling (TUNEL) to demonstrate apoptotic cells. We also analysed the cytokine gene transcription via real\u2010time quantitative reverse transcription PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The NB\u2010UVB MEDs caused mild inflammatory changes yet no severe epidermal exfoliations in the irradiated skin. In DNCB\u2010treated skin irradiated by the NB\u2010UVB MEDs, TUNEL\u2010positive dermal apoptotic cells were increased significantly compared with those of DNCB\u2010treated, nonirradiated skin. <jats:italic>INF\u2010\u03b3<\/jats:italic> and <jats:italic>TNF\u2010\u03b1<\/jats:italic> transcription levels in DNCB\u2010treated, irradiated skin were significantly lower than those in the DNCB\u2010treated, nonirradiated skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and clinical relevance<\/jats:title><jats:p>Phototherapy using NB\u2010UVB MEDs attenuated cutaneous Th1 and Tc1 cytokine responses with minimal skin damage in a canine model of hapten\u2010induced contact dermatitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13035","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T06:36:49Z","timestamp":1637303809000},"page":"605","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Narrow\u2010band ultraviolet B therapy attenuates cutaneous T\u2010cell responses in hapten\u2010induced, experimental contact dermatitis in beagles"],"prefix":"10.1111","volume":"32","author":[{"given":"Saki","family":"Onishi\u2010Sakamoto","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiichi","family":"Makishi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Internal Medicine Tokyo University of Agriculture and Technology Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumoto","family":"Takami","sequence":"additional","affiliation":[{"name":"Ushio, Inc. Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8230-1960","authenticated-orcid":false,"given":"Ryota","family":"Asahina","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7525-510X","authenticated-orcid":false,"given":"Sadatoshi","family":"Maeda","sequence":"additional","affiliation":[{"name":"Faculty of Applied Biological Sciences Gifu University Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0494-9710","authenticated-orcid":false,"given":"Masahiko","family":"Nagata","sequence":"additional","affiliation":[{"name":"Synergy Animal Hospital Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"Moore","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine 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