{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T10:41:55Z","timestamp":1775472115489,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cytological examination of the skin is an important diagnostic technique in dermatology for disease diagnosis and monitoring of treatment. Impression smear and tape\u2010strip preparation are gold standard cytological techniques. This study introduces a novel cytological method, slurry preparation, in which debris collected from the skin surface is macerated in warm sterile water and dried on the slide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>To compare organism yield between a novel and two standard cytological collection techniques by evaluating bacteria and <jats:italic>Malassezia<\/jats:italic> yeast counts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Thirty client\u2010owned dogs diagnosed with atopic dermatitis and lesions consistent with pyoderma and\/or <jats:italic>Malassezia<\/jats:italic> dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In a prospective, blinded comparison study, dermatological lesions from each dog were sampled using impression smear, tape\u2010strip and slurry preparation methodologies. Ten random reticle fields per lesion, accounting for a total area of 6.25\u2009\u00d7\u200910<jats:sup>\u22123<\/jats:sup>\u2009mm<jats:sup>2<\/jats:sup> (equivalent to \u02dc5% of a typical \u00d740 field), were evaluated by light microscopy for each cytological method. Bacterial and <jats:italic>Malassezia<\/jats:italic> organisms were measured as counts\/sample. The slurry preparation was compared to each of the standard methodologies separately using paired Student\u2019s <jats:italic>t<\/jats:italic>\u2010tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The slurry preparation was demonstrated to be more sensitive than gold standard techniques in detecting bacteria, with mean differences of 12.7 and 13.5 additional bacteria per cytological sample, compared to the impression and tape\u2010strip preparations, respectively. The slurry preparation did not differ significantly in detecting <jats:italic>Malassezia<\/jats:italic> organisms compared with impression smear preparation. The tape\u2010strip preparation was demonstrated to be more sensitive than the slurry preparation in the detection of <jats:italic>Malassezia<\/jats:italic> organisms, finding a mean difference of 0.22 more organisms per sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Slurry preparation presents advantages over the gold standard techniques in the detection of bacteria which can help to manage canine pyoderma more appropriately. Performing a slurry preparation in cases of suspected canine pyoderma could be considered as an alternative to the gold standard techniques.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13036","type":"journal-article","created":{"date-parts":[[2021,11,4]],"date-time":"2021-11-04T06:39:25Z","timestamp":1636007965000},"page":"108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel cytological technique for bacterial detection on canine skin"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6113-9394","authenticated-orcid":false,"given":"Naomi","family":"Rich","sequence":"first","affiliation":[{"name":"Animal Skin and Allergy Clinic 16429 7th Place West Lynnwood WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8284-9881","authenticated-orcid":false,"given":"Jourdan","family":"Brune","sequence":"additional","affiliation":[{"name":"Department of Comparative Medicine Magnuson Health Sciences Building The University of Washington Campus Box\u2009357340 Seattle WA 98195\u20107340 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Duclos","sequence":"additional","affiliation":[{"name":"Animal Skin and Allergy Clinic 16429 7th Place West Lynnwood WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"626","volume-title":"Clinical Veterinary Advisor: Dogs and Cats","author":"C\u00f4t\u00e9 E","year":"2015"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12138"},{"key":"e_1_2_7_4_1","first-page":"625","article-title":"Cutaneous cytology and the dermatology patient","volume":"58","author":"Bajwa J","year":"2017","journal-title":"Can Vet J"},{"key":"e_1_2_7_5_1","first-page":"28","article-title":"Biology, diagnosis and treatment of Malassezia dermatitis in dogs and cats Clinical Consensus Guidelines of the World Association for Veterinary Dermatology","volume":"31","author":"Bond R","year":"2020","journal-title":"Vet Dermatol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.2002.00308.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700403"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctsap.2006.05.004"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4160-5663-8.00002-1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.5326\/JAAHA-MS-6458"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2012.09.004"},{"key":"e_1_2_7_12_1","first-page":"78","volume-title":"Muller and Kirk\u2019s Small Animal Dermatology","author":"Miller WH","year":"2013"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3758\/BF03193146"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3758\/BRM.41.4.1149"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.13036","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vde.13036","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vde.13036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T23:24:31Z","timestamp":1693005871000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vde.13036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1111\/vde.13036"],"URL":"https:\/\/doi.org\/10.1111\/vde.13036","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]},"assertion":[{"value":"2021-07-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}