{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T21:49:03Z","timestamp":1780350543801,"version":"3.54.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2017DI037"],"award-info":[{"award-number":["2017DI037"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Staphylococcus pseudintermedius<\/jats:italic> is the main aetiological agent of canine pyoderma. Whole genome sequencing is the most comprehensive way of obtaining relevant genomic information about micro\u2010organisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/Objectives<\/jats:title><jats:p>Oxford Nanopore technology enables quality sequencing and <jats:italic>de novo<\/jats:italic> assembly of the whole genome of <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic>. Whole genome analysis of <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic> may help to better understand the pathogenesis of canine pyodermas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Twenty\u2010two strains of <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic> isolated from the skin of five healthy dogs and 33 strains isolated from skin of 33 dogs with pyoderma were analysed. DNA was extracted and sequenced using Oxford Nanopore MinION, a new technology that delivers longer reads in a hand\u2010held device. The pangenome was analysed and visualised with <jats:sc>Anvi<\/jats:sc>\u2019<jats:sc>o<\/jats:sc> 6.1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nanopore technology allowed the sequencing and <jats:italic>de novo<\/jats:italic> assembly of the genomes of 55 <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic> strains isolated from healthy dogs and from dogs with pyoderma. The average genome size of <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic> was 2.62\u2009Mbp, with 48% being core genome. Pyoderma isolates contained a higher number of antimicrobial resistance genes, yet the total number of virulence factors genes did not change between isolates from healthy dogs and from dogs with pyoderma. Genomes of meticillin\u2010resistant <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic> (MRSP) strains were larger than those of meticillin\u2010susceptible (MSSP) strains (2.80\u2009Mbp versus 2.59\u2009Mbp), as a consequence of a greater presence of antimicrobial resistance genes, phages and prophages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical importance<\/jats:title><jats:p>This technique allows much more precise and easier characterisation of canine <jats:italic>S<\/jats:italic>.\u2009<jats:italic>pseudintermedius<\/jats:italic> populations and may lead to a better understanding of the pathogenesis of canine pyodermas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13040","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T06:36:49Z","timestamp":1637303809000},"page":"654-663","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Whole genome sequencing and <i>de novo<\/i> assembly of <i>Staphylococcus pseudintermedius<\/i>: a pangenome approach to unravelling pathogenesis of canine pyoderma"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1537-0074","authenticated-orcid":false,"given":"Llu\u00eds","family":"Ferrer","sequence":"first","affiliation":[{"name":"Departament de Medicina i Cirurgia Animals Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4176-3402","authenticated-orcid":false,"given":"Roc\u00edo","family":"Garc\u00eda\u2010Fonticoba","sequence":"additional","affiliation":[{"name":"Departament de Medicina i Cirurgia Animals Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7301-4791","authenticated-orcid":false,"given":"Daniel","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Servei Veterinari de Gen\u00e8tica Molecular Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0709-5236","authenticated-orcid":false,"given":"Joaquim","family":"Vi\u00f1es","sequence":"additional","affiliation":[{"name":"Servei Veterinari de Gen\u00e8tica Molecular Universitat Aut\u00f2noma de Barcelona Barcelona Spain"},{"name":"Vetgenomics, PRUAB, Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6232-6992","authenticated-orcid":false,"given":"Norma","family":"F\u00e0bregas","sequence":"additional","affiliation":[{"name":"Vetgenomics, PRUAB, Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8852-2062","authenticated-orcid":false,"given":"Sergi","family":"Madro\u00f1ero","sequence":"additional","affiliation":[{"name":"Servei Veterinari de Gen\u00e8tica Molecular Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2772-6410","authenticated-orcid":false,"given":"Gabriele","family":"Meroni","sequence":"additional","affiliation":[{"name":"Department of Biomedical Surgical and Dental Sciences \u2013 One Health Unit Via Pascal 36 Milano 20133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0108-638X","authenticated-orcid":false,"given":"Piera A.","family":"Martino","sequence":"additional","affiliation":[{"name":"Department of Biomedical Surgical and Dental Sciences \u2013 One Health Unit Via Pascal 36 Milano 20133 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0204-9639","authenticated-orcid":false,"given":"Sof\u00eda","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Hospital Escuela de Peque\u00f1os Animales (HEPA) Centro de Investigaci\u00f3n Veterinaria de Tandil (CIVETAN) UNCPBA\u2010CONICET\u2010CICPBA Grupo de Medicina Veterinaria Traslacional (MEVET) Facultad de Ciencias Veterinarias Universidad Nacional del Centro de la Provincia de Buenos Aires (FCV\u2010UNCPBA) Tandil Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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