{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T10:45:03Z","timestamp":1747392303832,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T00:00:00Z","timestamp":1639008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cytokines are capable of manipulating the tumour microenvironment supporting tumour growth. Interleukin (IL)\u20108 and monocyte chemoattractant protein (MCP)\u20101, shown to be produced by various tumours, can negatively affect prognosis. The production of cytokines by canine mast cell tumours (MCT) has not been reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis\/objectives<\/jats:title><jats:p>We hypothesise that MCT cell lines produce IL\u20108 and\/or MCP\u20101 in addition to other cytokines, and that their production can be modulated by the Janus kinase (JAK) inhibitor oclacitinib. This pilot study aims to investigate the production of IL\u20108, MCP\u20101 and nine additional cytokines in three canine MCT cell lines, and determine the effects of oclacitinib on their production.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and materials<\/jats:title><jats:p>Reverse transcriptase\u2010PCR was used to detect the expression of IL\u20108 and MCP\u20101 mRNA in three MCT cell lines (CoMS, CM\u2010MC1 and VI\u2010MC1). The supernatant of the cell lines was evaluated for the presence of 11 cytokines [IL\u20102, \u20106, \u20107, \u20108, \u201010, \u201015 and \u201018, and MCP\u20101, granulocyte\u2010macrophage colony\u2010stimulating factor (GM\u2010CSF), interferon (IFN)\u03b3 and tumour necrosis factor (TNF)\u03b1] by enzyme\u2010linked immunosorbent assay (ELISA). The IC<jats:sub>50<\/jats:sub> of oclacitinib was identified for each cell line. ELISA was performed again to compare changes in IL\u20108 and MCP\u20101 in treated cell lines versus untreated controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Interleukin\u20108 and MCP\u20101 were produced by all MCT cell lines tested. Oclacitinib significantly decreased the release of IL\u20108 in the CoMS cell line and of MCP\u20101 in CoMS and VI\u2010MC1 in clinically relevant concentrations. Furthermore, oclacitinib significantly decreased the proliferation of all three cell lines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Interleukin\u20108 and MCP\u20101 are produced by canine MCT cell lines. Modulation of their production is possible with oclacitinib.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vde.13046","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T11:27:44Z","timestamp":1639049264000},"page":"159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cytokine production and the effects of oclacitinib in three canine mast cell tumour cell lines"],"prefix":"10.1111","volume":"33","author":[{"given":"Carlos H.","family":"de Mello Souza","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine University of Florida 2015 SW 16th Ave Gainesville FL 32610\u20100144 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0638-8961","authenticated-orcid":false,"given":"Keijiro","family":"Shiomitsu","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine University of Florida 2015 SW 16th Ave Gainesville FL 32610\u20100144 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine University of Florida 2015 SW 16th Ave Gainesville FL 32610\u20100144 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